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ZAP70 抗体 (C-Term)

Name: ZAP70 抗体 (C-Term)
SKU: ABIN238530
Availability: OutOfStock

This anti-ZAP70 antibody is a 小鼠单克隆 antibody detecting ZAP70 in WB, FACS 和 IF. Suitable for 人.

Western Blotting (WB) image for anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (C-Term) antibody (ABIN238530) (ZAP70 抗体 (C-Term))

2 images

产品编号 ABIN238530

发货至: 中国

Quick Overview for ZAP70 抗体 (C-Term) (ABIN238530)

抗原

See all ZAP70 抗体

ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This ZAP70 antibody is un-conjugated

应用范围

Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

克隆位点

ZAP-03

Discover our top product ZAP70 Primary Antibody

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C-Term

特异性

The antibody ZAP-03 reacts with ZAP70, a 70 kDa protein tyrosine kinase expressed in T and NK cells. ZAP70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.

产品特性

Synonyms: ZAP-70, ZAP 70, SRK, 70 kDa zeta-associated protein, Syk-related tyrosine kinase

纯化方法

Protein A affinity chromatography.

免疫原

Bacterially expressed fusion protein representing C-terminal part (160 amino acids) ofhuman ZAP-70.

亚型

IgG1
anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (C-Term) antibody
ZAP70 适用: 人 WB, ICC, ICFC 宿主: 小鼠 Monoclonal ZAP-03 unconjugated

anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr319) antibody
ZAP70 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 247-382) antibody
ZAP70 适用: 人 IHC, FACS, ELISA, IF, StM, Coat 宿主: 小鼠 Monoclonal ZAP70-2035 unconjugated

anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 169-390) antibody
ZAP70 适用: 人 WB, FACS, ELISA 宿主: 小鼠 Monoclonal 1F3G9 unconjugated

anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 337-600) antibody
ZAP70 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 1-254) antibody
ZAP70 适用: 人 IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal 2F3-2 unconjugated

anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (pTyr493) antibody
ZAP70 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 169-390) antibody
ZAP70 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 6D9C2 unconjugated

anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 247-382) antibody
ZAP70 适用: 人 IHC, ELISA, StM, Coat 宿主: 小鼠 Monoclonal ZAP70-2046 unconjugated

anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) (AA 1-300) antibody
ZAP70 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

应用备注

Flow Cytometry (2-5 μg/mL): Intracellular staining. Positive control: HPB-ALL human peripheral blood T cell leukemia cell line.

限制

仅限研究用
浓度

1.0 mg/mL

缓冲液

PBS, pH 7.4 containing 15 mM sodium azide as preservative.

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C/-20 °C

储存方法

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

有效期

12 months
抗原

ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

别名

ZAP70

背景

The ZAP70 (zeta-associated protein of 70 kDa) tyrosine kinase was identified as a tyrosine phosphoprotein that associates with TCR zeta subunit and undergoes tyrosine phosphorylation following TCR stimulation. ZAP70 is a Syk family tyrosine kinase primarily expressed in T and NK cells that plays an essential role in signaling through the TCR. TCR-mediated activation of T cells is crucial to the immune response. In humans, ZAP70 gene mutations resulting in lower ZAP70 protein expression levels or expression of catalytically inactive ZAP70 proteins, have been identified. ZAP70 deficiency results in the absence of mature CD8+ T cells and the prevention of TCR-mediated activation of CD4+ T cells, and it can lead to severe combined immunodeficiency. In patients with chronic lymphocytic leukemia (B-CLL), ZAP70 expression on B cell was shown to be correlated with disease progression and survival. ZAP70 is cytosolic protein migrating at 70 kDa in SDS-PAGE. It contains two N-terminal SH2 domains (Src homology domain 2) and a C-terminal kinase domain. Crystal structure of the ZAP70 SH2 domains in complex with a TCR zeta subunit peptide was described. During T cell activation , the binding of ZAP70 SH2 domains to the phosphorylated zeta subunit on the activated TCR complex causes a colocalization with the Lck tyrosine kinase that phosphorylates ZAP70 on Tyr493 in the activation loop. ZAP70 autophosphorylates multiple tyrosines in the region between the SH2 domains and the kinase domain, including the binding sites for additional SH2-containing signaling proteins such as SLP76, LAT, Lck, PLCgamma1, Vav, Shc, Ras-GAP, and Abl. ZAP70-mediated activation of these downstream effectors leads to the release of intracellular calcium stores, and the transcription of interleukin-2 and other genes important for an immune response.Synonyms: 70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZAP 70, ZAP-70

基因ID

7535

UniProt

P43403

途径

TCR Signaling, Ubiquitin Proteasome Pathway