TUBG1 抗体 (C-Term)
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Quick Overview for TUBG1 抗体 (C-Term) (ABIN238522)
抗原
See all TUBG1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 434-449, C-Term
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特异性
- The antibody TU-30 recognizes C-terminal peptide sequence of gamma-Tubulin.
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产品特性
- Synonyms: Tubulin gamma-1 chain, Gamma-1-tubulin, GCP-1, Gamma-tubulin complex component 1
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纯化方法
- Protein-A Affinity Chromatography
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免疫原
- C-terminal peptide (aa 434-449) of gamma-tubulin counjugated to KLHAA Sequence: EYHAATRPDYISWGTQ
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亚型
- IgG1
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应用备注
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Immunocytochemistry (Purified Antibody: 1-2 μg/mL, Staining technique: (a) Fix cells for 10 min in methanol at -20C and for 6 min in acetone at -20C, (b) Fix cells directly inmethanol for 10 min at -20C or in acetone for 10 min at -20C. Incubation: 45 min in roomtemperature, Positive control: P-19 mouse embryonal carcinoma cell line 3T3 mousefibroblasts, Application note: The antibody TU-30 stains only fixed cells). Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH ~7.4 with 15 mM Sodium Azide as preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch. -
有效期
- 12 months
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- TUBG1 (Tubulin, gamma 1 (TUBG1))
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别名
- TUBG1 / Tubulin gamma 1
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背景
- The gamma-tubulin (TUBG1, relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01 % of tubulin dimer). The gamma-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing gamma-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface.Synonyms: GCP-1, Gamma-1-tubulin, Gamma-tubulin complex component 1, Tubulin gamma-1 chain
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基因ID
- 7283
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UniProt
- P23258
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途径
- Microtubule Dynamics, M Phase
抗原
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