CD300a 抗体
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北京 101111
Quick Overview for CD300a 抗体 (ABIN238465)
抗原
See all CD300a (CD300A) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody reacts with CD300a, a 60 kDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25 % of circulating T cells and 15 % of circulating B cells.
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交叉反应 （详细）
- Species reactivity (tested):Human
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产品特性
- Synonyms: CLM-8, CMRF35-H, CMRF35-H9, CMRF-35-H9, IRC1/IRC2, IRp60, CMRF35-like molecule 8,Inhibitory receptor protein 60, NK inhibitory receptor, CD300 antigen-like family member A
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纯化方法
- Protein A affinity chromatography.
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免疫原
- HPB human acute lymphoid leukemia cell line.
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亚型
- IgG1
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anti-CD300a (CD300A) (AA 18-180) antibody
CD300A 适用: 人 ELISA 宿主: 小鼠 Monoclonal 7H8E4 unconjugated
anti-CD300a (CD300A) (AA 18-180) antibodyCD300A 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD300a (CD300A) antibody (PE)CD300A 适用: 人 FACS 宿主: 小鼠 Monoclonal P192 PE
anti-CD300a (CD300A) antibodyCD300A 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal MEM-260 unconjugated
anti-CD300a (CD300A) (Internal Region) antibodyCD300A 适用: 人 WB, ICC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-CD300a (CD300A) (AA 1-299) antibodyCD300A 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-CD300a (CD300A) antibody (APC)CD300A 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-260 APC
anti-CD300a (CD300A) antibody (PE)CD300A 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-260 PE
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应用备注
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Flow Cytometry. Western Blotting (Non-reducing conditions): Positive material: HPB cell lineNegative material: JURKAT human cell line, RAJI human cell line. Sample preparation: Resuspend approx.50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysatewith non-reducing SDS-PAGE sample buffer.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.4 containing 15 mM sodium azide as preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. -
有效期
- 12 months
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- CD300a (CD300A)
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别名
- CD300A / CMRF35H
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背景
- CD300a (CMRF-35H, IRp60) is a non-MHC-specific inhibitory receptor of immunoglobulin superfamily, which contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that associate with SH2-containing phosphatases SHP-1 and SHP-2. CD300a is expressed on many cell types including T cells, NK cells, neutrophils, eosinophils or mast cells. Its triggering inhibits activating signals such as those of IL5, GM-CSF or eotaxin, as well as supresses mast cell degranulation or NK cell cytotoxic activity.Synonyms: CD300 antigen-like family member A, CLM-8, CMRF-35-H9, CMRF35-H, CMRF35-H9, CMRF35-like molecule 8, IRC1/IRC2, IRp60, Inhibitory receptor protein 60, NK inhibitory receptor
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基因ID
- 11314
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UniProt
- Q9UGN4
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途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
抗原
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