Orf1 抗体
This anti- antibody is a 兔 多克隆 antibody detecting in WB. Suitable for Neisseria meningitidis.
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Quick Overview for Orf1 抗体 (ABIN238346)
抗原
Orf1
适用
Neisseria meningitidis
宿主
兔
克隆类型
多克隆
标记
非结合性
应用范围
Western Blotting (WB)
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纯化方法
- Protein-A affinity chromatography.
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免疫原
- Slices containing purified FrpD250-6xHis-tagged protein were excised from 10% SDS-PAGE gels, grinded, mixed with 1 mg/ml of aluminum hydroxide in PBS, and used to immunize a rabbit.
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应用备注
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Suitable for Western Blotting under Reducing conditions (0.2-1 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.4 with 15 mM sodium azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Orf1
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物质类
- Viral Protein
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背景
- Orf1 (FrpD) is a Neisseria meningitidis outer membrane lipoprotein. Subcellular localization and [3H]palmitic acid labeling in Escherichia coli revealed that FrpD is synthesized with a type II signal peptide for export across the cytoplasmic membrane and is, upon processing to a lipoprotein, sorted to the outer bacterial membrane.
抗原
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