Shows only the Scl-70 precipitate band when tested using double immunodiffusion against rabbit thymus extract. Specificity is verified by identity of precipitation with CDC-AF reference serum. The titer obtained will vary with the test system, however, undiluted antibody shows a strong Scl-70 precipitate band. Product shows no reactivity at a dilution of 1:100 when tested against purified SSA, SSB, Sm, Sm/RNp and Jo-1 antigen in an ELISA system designed to detect all IgG subclasses.
产品特性
Human anti Human Scl-70 Ag Human antibody to Human Scl-70 antigen
纯化方法
Single donor human plasma is delipidized and defibrinated. An immunoglobulin fraction is then precipitated and collected. Human plasma
Suitable for use in Immunodiffusion and ELISA. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitute with 2 mL deionized water.
缓冲液
Lyophilized from 0.01 M PBS, 0.11 M Sodium chloride, pH 7.2, 0.02 % Sodium azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.