alpha Tubulin 抗体 (Biotin)
Quick Overview for alpha Tubulin 抗体 (Biotin) (ABIN2380025)
抗原
See all alpha Tubulin (TUBA1) 抗体适用
宿主
克隆类型
标记
应用范围
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交叉反应
- 牛, 小鸡, 山羊, 人, 小鼠, 大鼠, 绵羊
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交叉反应 (详细)
- Calculated cross reactivity: Bo Ch Gt Hu Mo Rt Sh
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产品特性
- Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1) (Biotin)
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纯化方法
- Purified by immunoaffinity chromatography.
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免疫原
- Synthetic peptide corresponding to aa 425-451 at the C-Terminal of human alpha tubulin.
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亚型
- IgG
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应用备注
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ELISA: 1:20,000-1:100,000
Western Blot: 1:2000-1:10,000
Immunohistochemistry: 1:1000-1:5000
Optimal dilutions to be determined by the researcher. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute with 100ul sterile dH2O.
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缓冲液
- Supplied as a lyophilized powder in PBS, 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 10 mg/mL BSA, 0.01 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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- alpha Tubulin (TUBA1)
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别名
- Tubulin, alpha
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背景
- Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (A and B-tubulin). A and B-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric a-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated A-tubulin is referred to as G-tubulin. Another post-translational modification of detyrosinated a A tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This makes an excellent loading control.
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途径
- Microtubule Dynamics
抗原
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