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alpha Tubulin 抗体 (Biotin)

This anti-alpha Tubulin antibody is a 兔 多克隆 antibody detecting alpha Tubulin in WB, ELISA 和 IHC. Suitable for 人.
产品编号 ABIN2380025
发货至: 中国

Quick Overview for alpha Tubulin 抗体 (Biotin) (ABIN2380025)

抗原

See all alpha Tubulin (TUBA1) 抗体
alpha Tubulin (TUBA1)

适用

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宿主

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克隆类型

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多克隆

标记

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This alpha Tubulin antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
  • 交叉反应

    牛, 小鸡, 山羊, 人, 小鼠, 大鼠, 绵羊

    交叉反应 (详细)

    Calculated cross reactivity: Bo Ch Gt Hu Mo Rt Sh

    产品特性

    Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1) (Biotin)

    纯化方法

    Purified by immunoaffinity chromatography.

    免疫原

    Synthetic peptide corresponding to aa 425-451 at the C-Terminal of human alpha tubulin.

    亚型

    IgG
  • 应用备注

    ELISA: 1:20,000-1:100,000
    Western Blot: 1:2000-1:10,000
    Immunohistochemistry: 1:1000-1:5000
    Optimal dilutions to be determined by the researcher.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitute with 100ul sterile dH2O.

    缓冲液

    Supplied as a lyophilized powder in PBS, 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 10 mg/mL BSA, 0.01 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
  • 抗原

    alpha Tubulin (TUBA1)

    别名

    Tubulin, alpha

    背景

    Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (A and B-tubulin). A and B-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric a-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated A-tubulin is referred to as G-tubulin. Another post-translational modification of detyrosinated a A tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This makes an excellent loading control.

    途径

    Microtubule Dynamics
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