Suitable for immunocytochemical and Western blot detection of the D2s dopamine receptor. This antiserum has been found to stain specific cells in various regions of PLP fixed rat brain sections at 1:500 dilution. This includes Medial septum, Nucleus accumbens, Dentate gyrus, Globus pallidus, Medial forebrain bundle, Cortex regions 1-3, Substantia Nigra reticulata, and the Ventral tegmental area. Western immunoblots using whole rat brain homogenate resulted in a single band being detected at ~48kD at 1:800 dilution.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitute with 0.1 mL of PBS which contains 11 mg/mL BSA or with additional buffer for more dilute antisera.
储存液
Without preservative
储存条件
4 °C/-20 °C
储存方法
Store at 2-8 °C (preferably in a dessicator). Reconstituted: Aliquot and store at -28 °C.