Collagen Type V 抗体
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北京 101111
Quick Overview for Collagen Type V 抗体 (ABIN237044)
抗原
See all Collagen Type V (COL5) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Reacts with most mammalian Type V collagens and has negligible cross-reactivity with Type I, II, III, IV, and VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible. Typically less than 1% cross reactivity against other types of collagen was detected using ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.
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产品特性
- Rabbit anti Collagen Type V, Rabbit antibody to Human Collagen Type V
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纯化方法
- Immuno Affinity Chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
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免疫原
- Collagen Type V from human and bovine placenta
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anti-Collagen, Type V (COL5) antibody
COL5 适用: 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type V (COL5) (AA 1450-1500) antibodyCOL5 适用: 人, 大鼠, 小鼠 WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type V (COL5) antibodyCOL5 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type V (COL5) (AA 61-90), (N-Term) antibodyCOL5 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type V (COL5) antibody (Biotin)COL5 适用: 人 ELISA 宿主: 山羊 Polyclonal Biotin
anti-Collagen, Type V (COL5) antibody (Biotin)COL5 适用: 人, Cow WB, ELISA, IHC, IP 宿主: 兔 Polyclonal Biotin
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应用备注
- Have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve and for non-denaturing immunoblot for highly sensitive qualitative analysis. Was assayed by non-denaturing immunoblot and found to be reactive against Collagen V at a dilution of 1:5.000 to 1:10.000. This product was also assayed against 1.0 µg of Collagen V in a standard ELISA using Peroxidase conjugated Affinity Purified Goat anti-Rabbit IgG (H&L) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:4.000 to 1:8.000 of the stock concentration is suggested for this product. For immunohistochemistry on frozen tissue sections dilute the product 1:50 to 1:200. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, 0.01 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
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Avoid multiple freeze/thaw cycles.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use. -
储存条件
- 4 °C
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储存方法
- Upon receipt, store at 2-8 °C as an undiluted liquid. For long term storage, mix with an equal volume of glycerol, aliquot and store at -28 °C.
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有效期
- 12 months
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- Collagen Type V (COL5) (Collagen, Type V (COL5))
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别名
- Collagen Type V
抗原
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