Borrelia Burgdorferi 抗体 (FITC)
This anti-Borrelia Burgdorferi antibody is a 兔 多克隆 antibody detecting Borrelia Burgdorferi in WB, IF 和 IHC (p). Suitable for Borrelia burgdorferi.
Quick Overview for Borrelia Burgdorferi 抗体 (FITC) (ABIN236886)
抗原
Borrelia Burgdorferi
适用
Borrelia burgdorferi
宿主
兔
克隆类型
多克隆
标记
This Borrelia Burgdorferi antibody is conjugated to FITC
应用范围
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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特异性
- Reactive Western blot bands at 83kD, 41kD, 34kD and 31kD and additional low MW bands. Cross-reacts with Treponema pallidum, B. hermsii & B. parkerii.
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产品特性
- Rabbit anti B. Burgdorferi, Rabbit antibody to Borrelia burgdorferi (Lyme) Fluorescein conjugated
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纯化方法
- Covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.
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免疫原
- Whole cell preparation from B. burgdorferi.
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anti-Borrelia Burgdorferi antibody
适用: Borrelia burgdorferi WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Suitable for use in direct IFA, Western blot and immunohistochemistry (formalin fixed/paraffin). Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Protocol for direct IFA: 1. Multi-well slide contains acetone fixed organisms (BION Enterprises). 2. 2-fold dilutions of ABIN236886 starting at 1:10 out to 1:80 with a counterstain blocking diluents (Evan’s Blue in BSA solution). 3. Incubate 30 minutes at Room Temperature. 4. Wash 5. Mounting medium added to each well before coverslipping the slide. 6. Typically observe 4+ staining for several of the dilutions.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 0.01 M PBS, pH7.2 containing 10 mg/mL BSA, 0.1 % Sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
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Avoid multiple freeze/thaw cycles.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use. -
储存条件
- 4 °C/-20 °C
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储存方法
- Store (up to 6 months) at 2-8 °C under subdued light. Long term, aliquot and store at -28 °C.
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有效期
- 6 months
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- Borrelia Burgdorferi
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别名
- Borrelia Burgdorferi (Lyme)
抗原
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