Immunoblotting: 1 µg/mL HRP/ECL detection. Recommended blocking with casein/tween 20 based buffer. Western blot Analysis Amyloid beta A4 peptides were applied to SDS-PAGE and transferred to a PVDF membrane. The blot was probed with 2- g/mL Q67350M for 1 hour at 15-22-C and developed by ECL (exp. time: 30 sec.). Lane 1: bA4 (1-40) Lane 2: bA4 (1-42) Lane 3: bA4 (1-43) Immunocytochemistry: 1-10 µg/mL ELISA: 0.1 µg/mL Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitute with 1 mL water (15 minutes at room temperature)
缓冲液
Lyophilized from 2X PBS, PEG and Sucrose, 0.09 % Sodium azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C/-20 °C/-80 °C
储存方法
Store lyophilized product at -20 °C. After reconstitution, aliquot and store at -80 °C. Thawed aliquots may be stored (up to 3 months) at 2-0 °C.