IL17RA 抗体
(1 reference)
Quick Overview for IL17RA 抗体 (ABIN233847)
抗原
See all IL17RA 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-401-a49
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Supplier
- Rockland
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原理
- IL-7 Receptor Alpha Chain phospho Y449 Antibody
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交叉反应 （详细）
- This antibody is specific for mouse IL-7 protein phosphorylated at Y449.
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产品特性
- Synonyms: rabbit anti-IL-7 Receptor alpha pY449 antibody, rabbit anti-Interleukin-7 Receptor alpha pY449 antibody, IL7R alpha antibody, IL-7R alpha, IL 7R α, Interleukin7 Receptor, Interleukin 7 receptor alpha chain antibody, Interleukin 7 receptor α antibody
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纯化方法
- This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phospho-peptide coupled to agarose beads followed by solid phase adsorption against non-phospho-peptide.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near Y449 of mouse IL-7 protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Interleukin 17 Receptor A (IL17RA) (AA 590-848) antibody
IL17RA 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 17 Receptor A (IL17RA) (AA 33-320) antibodyIL17RA 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 6H1B1 unconjugated
anti-Interleukin 17 Receptor A (IL17RA) (AA 33-172) antibodyIL17RA 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 17 Receptor A (IL17RA) (AA 377-534) antibodyIL17RA 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 17 Receptor A (IL17RA) (AA 201-300) antibodyIL17RA 适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 17 Receptor A (IL17RA) (AA 552-743) antibodyIL17RA 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 17 Receptor A (IL17RA) (N-Term) antibodyIL17RA 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 17 Receptor A (IL17RA) (AA 33-320) antibodyIL17RA 适用: 人 ELISA, FACS 宿主: 兔 Monoclonal DM126 unconjugated
anti-Interleukin 17 Receptor A (IL17RA) (AA 697-866) antibodyIL17RA 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 17 Receptor A (IL17RA) (AA 617-847) antibodyIL17RA 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: This affinity purified antibody has been tested for use in ELISA, immunoprecipitation, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 55 kDa in size corresponding to phosphorylated IL-7 by western blotting in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with mouse IL-7 pY449 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
Western Blot Dilution: 1:10,000
ELISA Dilution: 1:5,000-1:50,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.82 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store IL-7 Receptor Alpha Chain phospho Y449 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Impaired CD8 T cell memory and CD4 T cell primary responses in IL-7R alpha mutant mice." in: The Journal of experimental medicine, Vol. 204, Issue 3, pp. 619-31, (2007) (PubMed).
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: "Impaired CD8 T cell memory and CD4 T cell primary responses in IL-7R alpha mutant mice." in: The Journal of experimental medicine, Vol. 204, Issue 3, pp. 619-31, (2007) (PubMed).
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- IL17RA (Interleukin 17 Receptor A (IL17RA))
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别名
- IL17R
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背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Interleukin-7 is a glycoprotein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R), the active receptor is an alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. The human and mouse sequence is nearly identical. In humans, severe combined immunodeficiency is caused by genetic defects in IL-7 receptor. The tyrosine residue at the 449 position is a critical signaling site of the intracellular domain of IL-7 receptor. This site is rapidly phosphorylated by janus kinases after the IL-7 receptor is engaged. IL-7 Receptor Alpha Chain phospho Y449 Antibody is ideal for investigators involved in Cell Signaling, Signal Transduction and Immunology research.
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基因ID
- 16197, 45269152
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UniProt
- P16872
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途径
- SARS-CoV-2 Protein Interactome
抗原
-
