TDGF1 抗体
(5 references)
Quick Overview for TDGF1 抗体 (ABIN233828)
抗原
See all TDGF1 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-401-997
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Supplier
- Rockland
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原理
- Cripto-1 TDGF1 Antibody
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交叉反应 （详细）
- This antibody is specific for human Cripto-1 protein.
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产品特性
- Synonyms: rabbit anti-Cripto 1 antibody, rabbit anti-Cripto-1 antibody, Cripto 1 growth factor antibody, Epidermal growth factor like cripto protein CR1 antibody, TDGF 1 antibody, Teratocarcinoma-derived growth factor 1, CRGF, TDGF1
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纯化方法
- This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human Cripto-1 protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Teratocarcinoma-Derived Growth Factor 1 (TDGF1) (AA 1-188) antibody
TDGF1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Teratocarcinoma-Derived Growth Factor 1 (TDGF1) antibodyTDGF1 适用: 人, 大鼠, 小鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Teratocarcinoma-Derived Growth Factor 1 (TDGF1) (AA 37-70), (N-Term) antibodyTDGF1 适用: 小鼠 WB, FACS 宿主: 兔 Polyclonal RB51515 unconjugated
anti-Teratocarcinoma-Derived Growth Factor 1 (TDGF1) (Internal Region) antibodyTDGF1 适用: 人, 大鼠, 小鼠 ELISA, WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Teratocarcinoma-Derived Growth Factor 1 (TDGF1) (AA 35-188) antibodyTDGF1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Teratocarcinoma-Derived Growth Factor 1 (TDGF1) (AA 1-188) antibodyTDGF1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Teratocarcinoma-Derived Growth Factor 1 (TDGF1) (Internal Region) antibodyVerified TDGF1 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Teratocarcinoma-Derived Growth Factor 1 (TDGF1) (AA 18-48), (N-Term) antibodyTDGF1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB3957 unconjugated
anti-Teratocarcinoma-Derived Growth Factor 1 (TDGF1) (N-Term) antibodyTDGF1 适用: 人 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 21 kDa in size corresponding to Cripto-1 by western blotting in the appropriate cell lysate or extract. Cripto-1 is reported as a 188 amino acid protein with a molecular weight of 21.1 kDa.
Western Blot Dilution: 1:1000
ELISA Dilution: 1:105,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.66 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Cripto-1 vaccination elicits protective immunity against metastatic melanoma." in: Oncoimmunology, Vol. 5, Issue 5, pp. e1128613, (2016) (PubMed).
: "Translational significance of Nodal, Cripto-1 and Notch4 in adult nevi." in: Oncology letters, Vol. 12, Issue 2, pp. 1349-1354, (2016) (PubMed).
: "Age-Dependent Association between Protein Expression of the Embryonic Stem Cell Marker Cripto-1 and Survival of Glioblastoma Patients." in: Translational oncology, Vol. 6, Issue 6, pp. 732-41, (2014) (PubMed).
: "The significance of a Cripto-1 positive subpopulation of human melanoma cells exhibiting stem cell-like characteristics." in: Cell cycle (Georgetown, Tex.), Vol. 12, Issue 9, pp. 1450-6, (2013) (PubMed).
: "Cancer hallmarks in induced pluripotent cells: new insights." in: Journal of cellular physiology, Vol. 225, Issue 2, pp. 390-3, (2010) (PubMed).
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: "Cripto-1 vaccination elicits protective immunity against metastatic melanoma." in: Oncoimmunology, Vol. 5, Issue 5, pp. e1128613, (2016) (PubMed).
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- TDGF1 (Teratocarcinoma-Derived Growth Factor 1 (TDGF1))
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别名
- TDGF1
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背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Human Cripto-1 (CR-1), also known as Teratocarcinoma-derived growth factor 1 (TDGF1), is a member of the epidermal growth factor (EGF)-GFC family and has been implicated in both embryogenesis and carcinogenesis. During early vertebrate development, CR-1 functions as a co-receptor for Nodal, a transforming growth factor b (TGFb) family member, and is essential for mesoderm and endoderm formation and anterior-posterior and left-right axis establishment. In adult tissues, CR-1 is expressed at a low level in all stages of mammary gland development, and expression increases during pregnancy and lactation. Over-expression of CR-1 in mouse mammary epithelial cells leads to their transformation in vitro, and when injected in mammary glands, CR-1 produces ductal hyperplasias.
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基因ID
- 6997, 4507425
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UniProt
- P13385
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途径
- EGFR Signaling Pathway
抗原
-
