Feline Immunodeficiency Virus Matrix (MA) (FIV p15) (Internal Region) 抗体
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- 抗原
- Feline Immunodeficiency Virus Matrix (MA) (FIV p15)
- 抗原表位
- Internal Region
- 适用
- Feline Immunodeficiency Virus (FIV)
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
- 应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
- Supplier Product No.
- 600-401-992
- Supplier
- Rockland
- 原理
- FIV matrix protein p15 Antibody
- 交叉反应 （详细）
- This affinity purified antibody is directed against FIV Matrix Protein p15.
- 产品特性
- Synonyms: rabbit anti-Feline immunodeficiency virus matrix protein p15 antibody, FIV Matrix Protein p15, Capsid protein p24, p1, Nucleocapsid protein p13, Gag polyprotein, Core polyprotein
- 纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids from an internal region of FIV Matrix Protein p15.
Immunogen Type: Conjugated Peptide
- 亚型
- IgG
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anti-Feline Immunodeficiency Virus Matrix (MA) (FIV p15) (Internal Region) antibody
FIV p15 适用: Feline Immunodeficiency Virus (FIV) ELISA, WB, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting and suitable for immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15 kDa in size corresponding to FIV Matrix Protein p15 by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:1,000 - 1:10,000
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.22 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Feline Immunodeficiency Virus Matrix (MA) (FIV p15)
- 物质类
- Viral Protein
- 背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Feline immunodeficiency virus (FIV) belongs to the lentivirus family. This family is characterized by assembly of the viral capsid at either the plasma membrane or at the limiting membrane of late endosomes. The capsid assembles from the viral Gag polyprotein. Upon release of a budding virion, Gag precursor protein is cleaved by the viral protease into its mature products, namely Matrix Protein, Capsid and Nucleocapsid. Matrix Protein, located at the N-terminus of the Gag polyprotein, is usually myristylated during protein translation, prior to the later events of virus assembly. The myristate moiety is believed to be sequestered within the Matrix Protein during protein translation and later facilitates membrane binding upon exposure resulting from conformational changes. Essential functions attributed to the Matrix Protein of lentiviruses include targeting newly synthesized Gag precursor proteins to the site of virus assembly by binding with cellular components such as phosphatidylinositides. In the mature virus particle, the Matrix Protein provides internal structure to the virion within the capsid, but is not exposed at the surface of the particle. Based on studies with HIV, it is postulated that FIV Matrix Protein may also serve additional functions, including nuclear localization of the viral core upon entry of the virus into a new host cell.
- 基因ID
- 1489988, 120815
- UniProt
- P16087
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