AHSA1 抗体 (Internal Region)
(4 references)
Quick Overview for AHSA1 抗体 (Internal Region) (ABIN233816)
抗原
See all AHSA1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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Supplier Product No.
- 600-401-974
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Supplier
- Rockland
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原理
- AHA1 Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against human AHA1 protein.
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产品特性
- Synonyms: rabbit anti-AHA1 Antibody, Aha-1, Aha 1, Ahsa1 antibody, Activator of Hsp90 ATPase, Activator of 90 kDa heat shock protein ATPase homolog 1 antibody
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human AHA1 protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Activator of HSP90 ATPase Activity 1 (AHSA1) (AA 1-338) antibody
AHSA1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1A2-A8 unconjugated
anti-Activator of HSP90 ATPase Activity 1 (AHSA1) (AA 1-338) antibodyAHSA1 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 4F3-B6 unconjugated
anti-Activator of HSP90 ATPase Activity 1 (AHSA1) antibody (Atto 488)AHSA1 适用: 小鼠 WB, ELISA, IHC, IP, IF, ICC 宿主: 大鼠 Monoclonal 25F2-D9 Atto 488
anti-Activator of HSP90 ATPase Activity 1 (AHSA1) antibody (Atto 390)AHSA1 适用: 小鼠 WB, ELISA, IHC, IP, IF, ICC 宿主: 大鼠 Monoclonal 25F2-D9 Atto 390
anti-Activator of HSP90 ATPase Activity 1 (AHSA1) antibodyAHSA1 适用: 小鼠 WB, ELISA, IHC, IP, IF, ICC 宿主: 大鼠 Monoclonal 25F2-D9 unconjugated
anti-Activator of HSP90 ATPase Activity 1 (AHSA1) antibody (HRP)AHSA1 适用: 小鼠 WB, ELISA, IHC, IP, IF, ICC 宿主: 大鼠 Monoclonal 25F2-D9 HRP
anti-Activator of HSP90 ATPase Activity 1 (AHSA1) (Center) antibodyAHSA1 适用: 人, 小鼠 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Activator of HSP90 ATPase Activity 1 (AHSA1) antibody (FITC)AHSA1 适用: 小鼠 WB, ELISA, IHC, IP, IF, ICC 宿主: 大鼠 Monoclonal 25F2-D9 FITC
anti-Activator of HSP90 ATPase Activity 1 (AHSA1) antibody (Biotin)AHSA1 适用: 小鼠 WB, ELISA, IHC, IP, IF, ICC 宿主: 大鼠 Monoclonal 25F2-D9 Biotin
anti-Activator of HSP90 ATPase Activity 1 (AHSA1) antibody (PE)AHSA1 适用: 小鼠 WB, ELISA, IHC, IP, IF, ICC 宿主: 大鼠 Monoclonal 25F2-D9 PE
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应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38-40 kDa in size corresponding to AHA1 protein by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1 μg/mL
ELISA Dilution: 1:35,000 - 1:185,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.08 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Hsp90 middle domain phosphorylation initiates a complex conformational program to recruit the ATPase-stimulating cochaperone Aha1." in: Nature communications, Vol. 10, Issue 1, pp. 2574, (2019) (PubMed).
: "The FNIP co-chaperones decelerate the Hsp90 chaperone cycle and enhance drug binding." in: Nature communications, Vol. 7, pp. 12037, (2018) (PubMed).
: "Phosphorylation induced cochaperone unfolding promotes kinase recruitment and client class-specific Hsp90 phosphorylation." in: Nature communications, Vol. 9, Issue 1, pp. 265, (2018) (PubMed).
: "Client Proteins and Small Molecule Inhibitors Display Distinct Binding Preferences for Constitutive and Stress-Induced HSP90 Isoforms and Their Conformationally Restricted Mutants." in: PLoS ONE, Vol. 10, Issue 10, pp. e0141786, (2016) (PubMed).
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: "Hsp90 middle domain phosphorylation initiates a complex conformational program to recruit the ATPase-stimulating cochaperone Aha1." in: Nature communications, Vol. 10, Issue 1, pp. 2574, (2019) (PubMed).
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- AHSA1 (Activator of HSP90 ATPase Activity 1 (AHSA1))
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别名
- AHSA1
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背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Activator of Hsp90 ATPase (AHA1) stimulates the inherent ATPase cycle of Hsp90, which is essential for its chaperone activity in vivo. The activation and/or stability of many of the key regulatory and signaling proteins of the eukaryotic cell depend on their interaction with the Hsp90 Molecular chaperone. Hsp90 is assisted and regulated by co-chaperones that participate in an ordered series both to assist client-protein recruitment or release and to modulate progress through the ATPase coupled chaperone cycle. Structural analysis and mutagenesis show that binding of the N-terminal domain of AHA1 to Hsp90 promotes a conformational switch in the middle-segment catalytic loop (aa 370-390) of Hsp90 that exposes the catalytic Arg380 and enables its interaction with ATP in the N-terminal nucleotide-binding domain of the chaperone. Recent studies show that AHA1 modulates Hsp90-dependent stability of the folding of the cystic fibrosis transmembrane conductance regulator (CFTR) in the endoplasmic reticulum (ER). Down-regulation of AHA1 rescues misfolding of CFTR in cystic fibrosis.
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基因ID
- 10598
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NCBI登录号
- NP_001308370
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UniProt
- O95433
抗原
-
