SEPHS2 抗体
(1 reference)
Quick Overview for SEPHS2 抗体 (ABIN233801)
抗原
See all SEPHS2 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-401-999
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Supplier
- Rockland
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原理
- Selenophosphate Synthetase 2 Antibody
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交叉反应 （详细）
- This antibody reacts with mouse SPS2 and shows partial cross-reactivity with SPS1.
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产品特性
- Synonyms: rabbit anti-Selenophosphate Synthetase 2 Antibody, rabbit anti-SPS 2 antibody, Selenide water dikinase 2 antibody, Selenium donor protein 2 antibody, SEPHS2 antibody
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纯化方法
- This product was purified by Protein A chromatography from monospecific antiserum.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a full-length recombinant protein corresponding to mouse SPS2.
Immunogen Type: Recombinant Protein
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亚型
- IgG
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anti-Selenophosphate Synthetase 2 (SEPHS2) (AA 61-150) antibody
SEPHS2 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2G9 unconjugated
anti-Selenophosphate Synthetase 2 (SEPHS2) antibodySEPHS2 适用: 人 WB, IHC (p), ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Selenophosphate Synthetase 2 (SEPHS2) (Middle Region) antibodySEPHS2 适用: 人 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This Protein A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kDa in size corresponding to SPS2 by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500 - 1:2,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 2.3 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Competition between the Brain and Testes under Selenium-Compromised Conditions: Insight into Sex Differences in Selenium Metabolism and Risk of Neurodevelopmental Disease." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 46, pp. 15326-38, (2015) (PubMed).
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: "Competition between the Brain and Testes under Selenium-Compromised Conditions: Insight into Sex Differences in Selenium Metabolism and Risk of Neurodevelopmental Disease." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 46, pp. 15326-38, (2015) (PubMed).
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- SEPHS2 (Selenophosphate Synthetase 2 (SEPHS2))
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别名
- Sephs2
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背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Selenophosphate synthetase (SelD) catalyzes the conversion of selenium to selenophosphate which is required by a number of bacterial, archaeal and eukaryotic organisms for synthesis of selenocysteine-tRNA, the precursor of selenocysteine in selenoenzymes. A second selenophosphate synthetase (SPS2) was identified in mammals. SPS2 is itself a selenoprotein in mammals.
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基因ID
- 20768, 14717785
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UniProt
- P97364
抗原
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