MHV NSP9 抗体
The 小鼠 单克隆 anti-MHV NSP9 antibody (Clone 2C6-H1) (ABIN233751) specifically detects MHV NSP9 in WB 和 FM.
The antibody is reactive with Mouse Hepatitis Virus (MHV) samples.
产品编号 ABIN233751
Supplier Product No.: 200-301-a56
发货至:
中国
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Quick Overview for MHV NSP9 抗体 (ABIN233751)
抗原
MHV NSP9
适用
Mouse Hepatitis Virus (MHV)
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Western Blotting (WB), Fluorescence Microscopy (FM)
克隆位点
2C6-H1
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Supplier Product No.
- 200-301-a56
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Supplier
- Rockland
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产品特性
- Concentration Definition: by UV absorbance at 280 nm
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免疫原
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This antibody was produced in mice by repeated immunizations with E.coli derived full-length MHV-A59 nsp9 protein. This protein is part of the viral replicase polyprotein.
Immunogentype:Native -
亚型
- IgG
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anti-MHV NSP9 antibody
适用: SARS Coronavirus-2 (SARS-CoV-2) WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- This antibody has been tested for use in immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13 kDa in size corresponding to mature MHV-A59 nsp9 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells were grown on glass slides followed by infection with MHV-A59 strain and fixation with PBS/3%PFA. After washing and permeabilization of the fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 2.1 mg/mL
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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- MHV NSP9
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物质类
- Viral Protein
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背景
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The nonstructural protein 9 (nsp9) is one of the Mouse hepatitis virus replicase cleavage products, encoded by ORF1a. Nsp9 is an RNA-binding protein that is thought to be part of the viral replication complex, which is associated with intracellular membranes.
Synonyms: MHV-A59 nsp9 protein antibody -
基因ID
- 1489749, 25121567
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UniProt
- P0C6V0
抗原
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