MHV NSP9 抗体
This anti- antibody is a 小鼠 单克隆 antibody detecting in WB 和 FM. Suitable for Mouse Hepatitis Virus (MHV).
Quick Overview for MHV NSP9 抗体 (ABIN233751)
抗原
MHV NSP9
适用
Mouse Hepatitis Virus (MHV)
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Western Blotting (WB), Fluorescence Microscopy (FM)
克隆位点
2C6-H1
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Supplier Product No.
- 200-301-a56
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Supplier
- Rockland
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产品特性
- Concentration Definition: by UV absorbance at 280 nm
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免疫原
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This antibody was produced in mice by repeated immunizations with E.coli derived full-length MHV-A59 nsp9 protein. This protein is part of the viral replicase polyprotein.
Immunogentype:Native -
亚型
- IgG
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anti-MHV NSP9 antibody
适用: SARS Coronavirus-2 (SARS-CoV-2) WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- This antibody has been tested for use in immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13 kDa in size corresponding to mature MHV-A59 nsp9 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells were grown on glass slides followed by infection with MHV-A59 strain and fixation with PBS/3%PFA. After washing and permeabilization of the fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 2.1 mg/mL
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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- MHV NSP9
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物质类
- Viral Protein
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背景
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The nonstructural protein 9 (nsp9) is one of the Mouse hepatitis virus replicase cleavage products, encoded by ORF1a. Nsp9 is an RNA-binding protein that is thought to be part of the viral replication complex, which is associated with intracellular membranes.
Synonyms: MHV-A59 nsp9 protein antibody -
基因ID
- 1489749, 25121567
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UniProt
- P0C6V0
抗原
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