DBH 抗体 (AA 40-57)
Quick Overview for DBH 抗体 (AA 40-57) (ABIN232869)
抗原
See all DBH 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 40-57
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特异性
- Recognizes Dopamine Beta Hydroxylase (DbH) N-terminal. By immunoblot the antibody specifically reacts with a single (or double) band (s) of M. W. 70-75000 in samples of SDS-solubilized, DTT-reduced human adrenal medulla subjected to SDS-PAGE. Species cross-reactivity: By immunoblot: human, monkey (slight), sheep, canine (slight), rat and bovine. Also shown to react with equine ganglia cells.
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预测反应
- Percent identity by BLAST analysis: Human, Gibbon (100%) Gorilla (94%) Marmoset (89%) Mouse, Rat, Elephant, Porcine, Bovine (83%).
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic peptide, C-SAPRESPLPYHIPLDPEG-amide, cloned from human (DbH) cDNA. Percent identity by BLAST analysis: Human, Gibbon (100%), Gorilla (94%), Marmoset (89%), Mouse, Rat, Elephant, Porcine, Bovine (83%).
Type of Immunogen: Synthetic peptide -
亚型
- IgG
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应用备注
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Approved: IHC (1:250 - 1:1000), WB (1:250 - 1:1000)
Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:250-1:1000. Immunohistochemistry: 1:250-1:1000. Staining was evaluated in the locus ceruleus of formaldehyde-fixed, cryopreserved human and monkey brain. Showed weak staining on both human and monkey. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- Dulbecco's PBS (divalent-deficient), 10 % glycerol, 10 mM HEPES, pH 7.2, 20 mM Tris-HCl, pH 7.6, 0.1 % PVP-40, 0.05 % Tween-20, 0.1 % sodium azide, 1 mg/mL BSA.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
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- DBH (Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH))
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别名
- DBH/Dopamine Beta Hydroxylase
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背景
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Name/Gene ID: DBH
Family: Monooxygenase
Synonyms: DBH, Dopamine beta-monooxygenase, DBM, Dopamine beta-hydroxylase -
基因ID
- 1621
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UniProt
- P09172
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途径
- Carbohydrate Homeostasis
抗原
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