CENPA 抗体 (AA 4-17)
Quick Overview for CENPA 抗体 (AA 4-17) (ABIN232755)
抗原
See all CENPA 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 4-17
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特异性
- Recognizes human CENP-A, Mr17kD. Broad species cross-reactivity predicted due to sequence conservation.
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预测反应
- Percent identity by BLAST analysis: Human, Gorilla, Monkey (100%) Gibbon, Rabbit (93%) Nectri (86%).
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic peptide (RRRSRKPEAPRRRS) corresponding to amino acids 4-17 of human CENP-A. KLH conjugated. Percent identity by BLAST analysis: Human, Gorilla, Monkey (100%), Gibbon, Rabbit (93%), Nectri (86%).
Type of Immunogen: Synthetic peptide - KLH conjugated -
亚型
- IgG
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应用备注
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Approved: WB
Usage: Suitable for use in Western Blot analysis. Western Blot Analysis: 1:200-1:1000 detected CENP-A in RIPA lysates from HeLa acid extracted histones. 20 μg control histones, were resolved by electrophoresis, transferred to nitrocellulose and probed with 2 μg/mL anti-CENP-A. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 5 mg/mL BSA, 0.05 % sodium azide, 30 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- CENPA (Centromere Protein A (CENPA))
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别名
- CENPA / CENP-A
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背景
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Name/Gene ID: CENPA
Synonyms: CENPA, Centromere autoantigen A, Centromere-specific histone, CenH3, Centromere protein A, Centromere protein A, 17 kDa, CENP-A, Centromere protein A (17kD) -
基因ID
- 1058
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UniProt
- P49450
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途径
- Chromatin Binding, Maintenance of Protein Location
抗原
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