GNAS 抗体 (AA 1028-1037)
Quick Overview for GNAS 抗体 (AA 1028-1037) (ABIN228262)
抗原
See all GNAS 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1028-1037
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特异性
- Recognizes both the large (MW 48-52kD) and small (MW43-45kD) forms of bovine Gs alpha. Does not cross-react with other G proteins.
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预测反应
- Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Seabass, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito (100%).
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纯化方法
- Protein A purified
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免疫原
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Synthetic peptide corresponding to the C-terminal region of bovine Gs alpha (RMHLRQYELL) (KLH). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Seabass, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito (100%).
Type of Immunogen: Synthetic peptide - KLH conjugated -
亚型
- IgG
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anti-GNAS Complex Locus (GNAS) (AA 42-188) antibody
GNAS 适用: 人 ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 2A2B7 unconjugated
anti-GNAS Complex Locus (GNAS) (N-Term) antibodyGNAS 适用: 人, 小鼠, 大鼠, 犬, Cow, 兔, 马, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-GNAS Complex Locus (GNAS) antibodyKO Validated GNAS 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-GNAS Complex Locus (GNAS) (AA 42-188) antibodyGNAS 适用: 人 ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 7G6G5 unconjugated
anti-GNAS Complex Locus (GNAS) (N-Term) antibodyGNAS 适用: 人, 小鼠, 大鼠, Cow, Pig, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-GNAS Complex Locus (GNAS) (AA 310-404) antibodyGNAS 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-GNAS Complex Locus (GNAS) (Internal Region) antibodyVerified GNAS 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-GNAS Complex Locus (GNAS) (AA 1-394) antibodyGNAS 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-GNAS Complex Locus (GNAS) (AA 287-315), (C-Term) antibodyGNAS 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB33001 unconjugated
anti-GNAS Complex Locus (GNAS) (AA 286-315), (C-Term) antibodyGNAS 适用: 人 WB, IF, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: ICC (1:100), IP, WB (1:500 - 1:1000)
Usage: Western Blot: 1:1000-1:2000 detected both forms of Gs alpha as a doublet in 20 μg of rat brain microsomal preparation. Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with LS-C24097 (1:1000). Proteins were visualized using a IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.Immunoprecipitation: 2 μL immunoprecipitates Gs alpha(45kD) from 500 μg of solubilized CHO and mouse brain membrane preparations.Immunocytochemistry: 1:200 detected Gs alpha in 3T3 cells fixed for 3 minutes with 100 % ice-cold acetone.Optimal dilutions to be determined by researcher. -
说明
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Target Species of Antibody: Bovine
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.4, 0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- avoid freeze thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- GNAS (GNAS Complex Locus (GNAS))
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别名
- GNAS
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背景
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Name/Gene ID: GNAS
Synonyms: GNAS, AHO, C20orf45, Extra large alphas protein, GSP, GNAS complex locus, GNASXL, NESP, PHP1B, PHP1C, PHP1A, POH, SCG6, XLalphas, GNAS1, GPSA, GSA, NESP55, Protein ALEX, Secretogranin VI -
基因ID
- 2778
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途径
- Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Embryonic Body Morphogenesis
抗原
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