Choline Acetyltransferase 抗体
Quick Overview for Choline Acetyltransferase 抗体 (ABIN224453)
抗原
See all Choline Acetyltransferase (CHAT) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes choline acetyltransferase (ChAT)-positive cells. Species cross-reactivity: Human, Rat and Porcine.
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纯化方法
- Purified
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免疫原
- Highly purified ChAT obtained from porcine brain
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亚型
- IgG1
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anti-Choline O-Acetyltransferase (CHAT) (AA 517-732) antibody
CHAT 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Choline O-Acetyltransferase (CHAT) (AA 331-630) antibodyCHAT 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Choline O-Acetyltransferase (CHAT) (AA 98-128) antibodyCHAT 适用: 人, 小鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Choline O-Acetyltransferase (CHAT) (AA 101-200) antibodyCHAT 适用: 人, 小鼠, 大鼠, 犬 ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Choline O-Acetyltransferase (CHAT) (AA 354-592) antibodyCHAT 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Choline O-Acetyltransferase (CHAT) (C-Term) antibodyVerified CHAT 适用: 小鼠, 大鼠 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Choline O-Acetyltransferase (CHAT) antibodyCHAT 适用: 人 WB, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Choline O-Acetyltransferase (CHAT) (AA 446-652) antibodyCHAT 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Choline O-Acetyltransferase (CHAT) antibodyMicroarray verified CHAT 适用: 人 IHC 宿主: 小鼠 Monoclonal MAB872084 unconjugated
anti-Choline O-Acetyltransferase (CHAT) (C-Term) antibodyCHAT 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: ELISA, IHC, IHC-Pfa (10 - 20 μg/mL), WB
Usage: Fixation: a) Human tissue: Fix by placing fresh (5-8 h post mortem) tissue in a 1:1 solution of 4 % paraformaldehyde/10 % formaldehyde for 1 week at 4 °C. Divide into tissue blocks (basal forebrain, septum and basal ganglia). Incubate the blocks in sucrose solutions of increasing concentration (10 %, 20 % and 30 %). Prepare 50um sections and store in a cryoprotective solution at -15 °C (3).b) Rat tissue: Brain and spinal cord marrow tissue (transcardial perfusion). Perform a transcardial perfusion initially with oxygen-saturated heparin solution (II) (100-200ml) and subsequently with paraformaldehyde/picric acid solution (III) (400ml). Carry out immediate tissue preparation and fix for a further 30 minutes in the same solution. Wash out the fixative with several fresh quantities of Buffer A. Prepare 50um sections. -
说明
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Target Species of Antibody: Pig
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.6, 0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- avoid freeze thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))
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别名
- CHAT
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背景
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Name/Gene ID: CHAT
Synonyms: CHAT, Choline acetylase, Choline acetyltransferase, Choline O-acetyltransferase, CMS1A2, CHOACTASE, CMS1A -
基因ID
- 1103
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UniProt
- P28329
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途径
- Skeletal Muscle Fiber Development
抗原
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