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Vimentin 抗体 (FITC)

The FITC-conjugated 小鼠 单克隆 anti-Vimentin antibody (ABIN222825) specifically detects Vimentin in WB, IHC, ELISA, IF, IHC (p) 和 IHC (fro). The antibody is reactive with 人, Cow, 猴, 小鸡 和 犬 samples.
产品编号 ABIN222825
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Quick Overview for Vimentin 抗体 (FITC) (ABIN222825)

抗原

See all Vimentin (VIM) 抗体
Vimentin (VIM)

适用

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人, Cow, 猴, 小鸡, 犬

宿主

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小鼠

克隆类型

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单克隆

标记

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This Vimentin antibody is conjugated to FITC

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
  • 特异性

    Highly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. This clone has turned out to be the most avid mab to vimentin. Polypeptide reacting: Mr 57000 intermediate filament protein (vimentin) of mesenchymal cells. Reactivities on Cultured RD cells, glioma cells, fibroblasts (SV-80), MDCK. Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma. Species cross-reactivity: Human, monkey, bovine, canine, chicken, amphibia.

    纯化方法

    Protein A purified

    免疫原

    Vimentin (purified from bovine lens).

    Type of Immunogen: Purified protein

    亚型

    IgG2a
  • 应用备注

    Approved: ELISA, IF, IHC, IHC-Fr, IHC-P, WB

    Usage: Suitable for use in Western Blot, ELISA, Immunofluorescence microscopy and Immunohistochemistry. Immunohistochemistry (frozen and paraffin-embedded tissue and cytological material): 1:10 with PBS,  pH 7.4, Incubation Time 1 hr at RT, extended with paraffin. Protease pretreatment is required prior to antibody application. Positive control: Cultured RD cells, glioma cells, fibroblasts (SV-80), MDCK. Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    说明

    Target Species of Antibody: Bovine

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    Supplied as a liquid.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    4°C or -20°C, Avoid freeze-thaw cycles.
  • 抗原

    Vimentin (VIM)

    别名

    Vimentin

    背景

    Name/Gene ID: VIM

    Synonyms: VIM, Vimentin

    基因ID

    7431

    UniProt

    P08670

    途径

    Caspase Cascade in Apoptosis
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