Vimentin 抗体 (FITC)
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北京 101111
Quick Overview for Vimentin 抗体 (FITC) (ABIN222825)
抗原
See all Vimentin (VIM) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Highly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. This clone has turned out to be the most avid mab to vimentin. Polypeptide reacting: Mr 57000 intermediate filament protein (vimentin) of mesenchymal cells. Reactivities on Cultured RD cells, glioma cells, fibroblasts (SV-80), MDCK. Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma. Species cross-reactivity: Human, monkey, bovine, canine, chicken, amphibia.
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纯化方法
- Protein A purified
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免疫原
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Vimentin (purified from bovine lens).
Type of Immunogen: Purified protein -
亚型
- IgG2a
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应用备注
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Approved: ELISA, IF, IHC, IHC-Fr, IHC-P, WB
Usage: Suitable for use in Western Blot, ELISA, Immunofluorescence microscopy and Immunohistochemistry. Immunohistochemistry (frozen and paraffin-embedded tissue and cytological material): 1:10 with PBS, pH 7.4, Incubation Time 1 hr at RT, extended with paraffin. Protease pretreatment is required prior to antibody application. Positive control: Cultured RD cells, glioma cells, fibroblasts (SV-80), MDCK. Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
说明
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Target Species of Antibody: Bovine
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- Supplied as a liquid.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- Vimentin (VIM)
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别名
- Vimentin
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背景
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Name/Gene ID: VIM
Synonyms: VIM, Vimentin -
基因ID
- 7431
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UniProt
- P08670
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途径
- Caspase Cascade in Apoptosis
抗原
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