HTR7 抗体 (AA 8-23)
Quick Overview for HTR7 抗体 (AA 8-23) (ABIN222308)
抗原
See all HTR7 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 8-23
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特异性
- Recognizes Serotonin Receptor 7. Species cross-reactivity: Mouse and rat.
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic peptide sequence corresponding to aa8 - 23 of the rat 5-HT7 receptor.
Type of Immunogen: Synthetic peptide -
亚型
- IgG
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应用备注
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Approved: IF, IHC (1:100 - 1:300), WB (1:100 - 1:250)
Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:100-1:250. Immunohistochemistry: 1:100-1:300 in PBS - Bn/Av-HRP Technique. Control Tissue: The antiserum demonstrates positive labeling of rat cortex and. hippocampus. Perfusion Fixation: Fixative-4 % paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4, 500 mL over approximately 20 minutes. Post Fixation: 4 % paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4, 500 mL over approximately 20 minutes. Post Fixation-1.5 hour at 4? C. in 4 % paraformaldehyde in 0.1 M phosphate buffer, pH 7.4. Note: Paraformaldehyde is a necessary component in fixation of serotonin. If needed, low levels of glutaraldehyde (0.10.3 %) may be used in conjunction with paraformaldehyde. Sections: 50um vibratome. Antibody dilution: 1:100-1:300 in PBS-Bn-SA/HRP Technique. Absorption control: Synthetic rat 5HT 7 receptor. Incubation on Tissue: 16-24 hours at 4?C. -
说明
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Target Species of Antibody: Rat
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Reconstitute in 100 μL of Sterile distilled water.
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浓度
- Lot specific
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缓冲液
- Supplied as a liquid
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- HTR7 (5-Hydroxytryptamine (serotonin) Receptor 7 (Adenylate Cyclase-Coupled) (HTR7))
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别名
- HTR7 / 5-HT7 Receptor
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背景
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Name/Gene ID: HTR7
Subfamily: Serotonin
Family: GPCR
Synonyms: HTR7, 5-HT7, 5-HT7 receptor, 5-HT-7, 5-hydroxytryptamine 7 receptor, 5HT7, 5-HT-X, 5-hydroxytryptamine receptor 7, 5HT7 Receptor, HT7, Serotonin 5-HT7 receptor, Serotonin receptor 7 -
基因ID
- 3363
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UniProt
- P34969
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途径
- JAK/STAT Signaling
抗原
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