Serotonin Receptor 5A 抗体 (AA 17-34)
Quick Overview for Serotonin Receptor 5A 抗体 (AA 17-34) (ABIN222307)
抗原
See all Serotonin Receptor 5A (HTR5A) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 17-34
-
特异性
- Recognizes Serotonin Receptor 5A. Species cross-reactivity: Mouse and rat.
-
纯化方法
- Immunoaffinity purified
-
免疫原
-
Synthetic peptide sequence corresponding to aa17-34 of the rat 5-HT5A receptor.
Type of Immunogen: Synthetic peptide -
亚型
- IgG
-
-
-
-
应用备注
-
Approved: IF, IHC (1:100 - 1:300), WB (1:100 - 1:250)
Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:100-1:250. Immunohistochemistry: 1:100-1:300 in PBS/0.03 % Triton X-100 - Bn/Av-HRP Technique. Control Tissue: The antiserum demonstrates positive labeling of rat cortex and hippocampus. Perfusion Fixation: Fixative-4 % paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4, 500 mL over approximately 20 minutes. Post Fixation: 4 % paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4, 500 mL over approximately 20 minutes. Post Fixation -1.5 hour at 4? C. in 4 % paraformaldehyde in 0.1 M phosphate buffer, pH 7.4. Note: Paraformaldehyde is a necessary component in fixation of serotonin. If needed, low levels of glutaraldehyde (0.10.3 %) may be used in conjunction with paraformaldehyde. Sections: 50um vibratome. Absorption control: Synthetic rat 5HT 5A receptor. Incubation on Tissue: 16-24 hours at 4?C. -
说明
-
Target Species of Antibody: Rat
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- Lot specific
-
缓冲液
- Supplied as a liquid.
-
注意事项
- Avoid freeze-thaw cycles.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
-
-
- Serotonin Receptor 5A (HTR5A)
-
别名
- HTR5A / 5-HT5A Receptor
-
背景
-
Name/Gene ID: HTR5A
Subfamily: Serotonin
Family: GPCR
Synonyms: HTR5A, 5-HT-5, 5-HT-5A, 5-HT5A, 5-HT5a receptor, 5-hydroxytryptamine5a, Serotonin 5-HT5a receptor, Serotonin 5a receptor, Serotonin receptor 5A -
基因ID
- 3361
-
UniProt
- P47898
-
途径
- JAK/STAT Signaling
抗原
-