RPS6 抗体 (pSer235, Ser236)
Quick Overview for RPS6 抗体 (pSer235, Ser236) (ABIN222236)
抗原
See all RPS6 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer235, Ser236
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特异性
- Detects endogenous levels of human ribosomal protein S6 only when phosphorylated at Ser235/236 (Mr 32kD). Does not detect ribosomal protein S6 phosphorylated at other sites. Species cross-reactivity: Mouse, rat and zebrafish. Species sequence homology: chicken and Xenopus 100 % .
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纯化方法
- Protein A purified
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免疫原
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Synthetic phosphopeptide corresponding to residues surrounding Ser235 and Ser236 of human ribosomal protein S6 (KLH coupled).
Type of Immunogen: Synthetic phospho-peptide -
亚型
- IgG
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应用备注
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Approved: ELISA, Flo (1:50), ICC, IF, IHC, IHC-P, IP (1:100), WB (1:1000)
Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Flow Cytometry. Western Blot: 1:1000, incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:100. Immunohistochemistry (Paraffin): 1:50. Immunocytochemistry (for IF): 1:50. Flow Cytometry: 1:50. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.
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储存液
- Without preservative
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- RPS6 (Ribosomal Protein S6 (RPS6))
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别名
- RPS6 / S6
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背景
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Name/Gene ID: RPS6
Synonyms: RPS6, 40S ribosomal protein S6, S6, Ribosomal protein S6, Phosphoprotein NP33 -
基因ID
- 6194
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UniProt
- P62753
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途径
- Carbohydrate Homeostasis, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
抗原
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