Muscarinic Acetylcholine Receptor M2 抗体
(2 references)-
- 抗原 See all Muscarinic Acetylcholine Receptor M2 (CHRM2) 抗体
- Muscarinic Acetylcholine Receptor M2 (CHRM2) (Cholinergic Receptor, Muscarinic 2 (CHRM2))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Muscarinic Acetylcholine Receptor M2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Functional Studies (Func), Immunoassay (IA)
- 过滤
- 0.2 μm filtered
- 克隆位点
- B8E5
- Top Product
- Discover our top product CHRM2 Primary Antibody
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anti-Cholinergic Receptor, Muscarinic 2 (CHRM2) antibody
CHRM2 适用: 人, 小鼠 WB, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 1424CT461-78-60 unconjugated
anti-Cholinergic Receptor, Muscarinic 2 (CHRM2) antibodyCHRM2 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Cholinergic Receptor, Muscarinic 2 (CHRM2) (AA 168-250) antibodyCHRM2 适用: 人, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), FACS, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cholinergic Receptor, Muscarinic 2 (CHRM2) (AA 381-466) antibodyCHRM2 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cholinergic Receptor, Muscarinic 2 (CHRM2) (AA 208-388) antibodyCHRM2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Cholinergic Receptor, Muscarinic 2 (CHRM2) (Cytoplasmic Domain) antibodyCHRM2 适用: 人, 小鼠, 大鼠, Cow, 犬, Pig, 猴, 豚鼠, 绵羊, 兔, Hamster, Bat IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cholinergic Receptor, Muscarinic 2 (CHRM2) (AA 251-350) antibodyCHRM2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4B5 unconjugated
anti-Cholinergic Receptor, Muscarinic 2 (CHRM2) (Internal Region) antibodyCHRM2 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Cholinergic Receptor, Muscarinic 2 (CHRM2) (AA 3-20) antibodyCHRM2 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Cholinergic Receptor, Muscarinic 2 (CHRM2) antibodyCHRM2 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year
- 限制
- 仅限研究用
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- 缓冲液
- PBS, containing 0.1 % bovine serum albumin.
- 储存条件
- 4 °C
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: "cGMP-mediated inhibition of cardiac L-type Ca(2+) current by a monoclonal antibody against the M(2) ACh receptor." in: American journal of physiology. Cell physiology, Vol. 281, Issue 4, pp. C1251-8, (2001) (PubMed).
: "Immunochemical and functional characterization of an agonist-like monoclonal antibody against the M2 acetylcholine receptor." in: European journal of biochemistry / FEBS, Vol. 251, Issue 3, pp. 659-66, (1998) (PubMed).
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: "cGMP-mediated inhibition of cardiac L-type Ca(2+) current by a monoclonal antibody against the M(2) ACh receptor." in: American journal of physiology. Cell physiology, Vol. 281, Issue 4, pp. C1251-8, (2001) (PubMed).
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- 抗原
- Muscarinic Acetylcholine Receptor M2 (CHRM2) (Cholinergic Receptor, Muscarinic 2 (CHRM2))
- 别名
- Muscarinic Acetylcholine Receptor m2 (CHRM2 产品)
- 别名
- HM2 antibody, AChR-M2 antibody, Chrm-2 antibody, M2 antibody, chrm2 antibody, Acm2 antibody, GPM2 antibody, cholinergic receptor muscarinic 2 antibody, cholinergic receptor, muscarinic 2, cardiac antibody, cholinergic receptor, muscarinic 2a antibody, cholinergic receptor, muscarinic 2 antibody, CHRM2 antibody, Chrm2 antibody, chrm2a antibody
- 背景
- The monoclonal antibody B8E5 recognizes human muscarinic acetylcholine receptor M2 (M2 receptor), a G protein-coupled cardiovascular receptor of ~55 kDa. This receptor is an integral membrane protein consisting of seven membrane spanning α-helices linked together by extra- and intracellular loops that form a pharmacophore pocket. Autoantibodies directed against cardiovascular G protein-coupled receptors functionally interfering with the target have been described in several cardiovascular diseases. The M2 receptor is the predominant sybtype of muscarinic receptors present in the heart of mammalian species. The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. 2+ Monoclonal antibody B8E5 inhibits the ß-adrenergic L-type Ca currents through activation of the muscarinic acetylcholine receptor M2. It suggests that the antibody acts not via the classical pathway of decreasing cAMP, but rather by increasing cGMP. Monoclonal antibody B8E5 acts by functional dimerization of the receptor resulting in stabilization of the constitutive active receptor dimers and paradoxically induces a small decrease in carbachol affinity for the M2 receptor. It recognizes the pentapeptide VRTVE (aa 168-172) corresponding to the N-terminal part of the second extracellular loop of the human M2 receptor. M2ACh-R Aliases Peptide VRTVEDGECYIQFFSNAAVTFGTAI(C): Human second extracellular loop residues 168-192 Immunogen Mouse IgG2a
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