Coli Elongation Factor Tu 抗体
适用: E. coli
IA, IF, WB
宿主: 小鼠
Monoclonal
9E2
unconjugated
(3 references)-
- 抗原
- Coli Elongation Factor Tu
- 适用
- E. coli
- 宿主
- 小鼠
- 克隆类型
- 单克隆
- 应用范围
- Immunoassay (IA), Immunofluorescence (IF), Western Blotting (WB)
- 交叉反应 （详细）
- Cross reactivity: Bacteroides fragilis : Yes, Streptococcus oralis : Yes, Bacillus subtilis : Yes, Mycobacterium tuberculosis Weak Pseudomonas aeruginosa : Yes, Burkholderia cepacia : Yes, Deinococcus sp. : Yes
- 过滤
- 0.2 μm filtered
- 克隆位点
- 9E2
- 亚型
- IgG2a
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- 应用备注
- For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
- 限制
- 仅限研究用
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- 缓冲液
- PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
- 有效期
- 12 months
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: "Immune evasion of the human pathogen Pseudomonas aeruginosa: elongation factor Tuf is a factor H and plasminogen binding protein." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 5, pp. 2979-88, (2007) (PubMed).
: "Conservation of the amino-terminal epitope of elongation factor Tu in eubacteria and Archaea." in: Microbiology (Reading, England), Vol. 144 ( Pt 8), pp. 2241-6, (1998) (PubMed).
: "An epitope of elongation factor Tu is widely distributed within the bacterial and archaeal domains." in: Journal of bacteriology, Vol. 177, Issue 1, pp. 11-9, (1995) (PubMed).
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: "Immune evasion of the human pathogen Pseudomonas aeruginosa: elongation factor Tuf is a factor H and plasminogen binding protein." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 5, pp. 2979-88, (2007) (PubMed).
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- 抗原
- Coli Elongation Factor Tu
- 背景
- The monoclonal antibody 900 recognizes elongation factor Tu (EF-Tu), a 43 kDa multifunctional protein present in Escherichia coli. EF-Tu is one of the most abundant proteins present in prokaryotes, representing about 5 % of the total cellular protein of E. coli. During protein biosynthesis, the elongation process, EF-Tu catalyzes the binding of each aminoacyl-tRNA to the ribosome. It also interacts with several macromolecules and guanine nucleotides, including EF-Ts, GDP, GTP, and some ribosomal proteins. Monoclonal antibody 900 recognizes EF-Tu in organisms belonging to bacterial and archaeal domains, yet no organisms from the eukaryotic domain. The panbacterial distribution of EF-Tu, which is present in large amounts in every prokaryotic cell, renders this protein a good candidate for diagnostic purposes. The highly conserved epitope recognized by monoclonal antibody 900 is located at the very end of the N-terminus of the EF-Tu molecule (SKEKFE). Aliases EF-TU, Elongation factor TUF, Elongation factor Tu Immunogen E. coli K-12 C600 Rif ( pKT146) bacteria
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