Shiga Toxin 2 (Stx-2) 抗体
Cited in 2+ publications.
The 小鼠 单克隆 anti-Shiga Toxin 2 antibody (Clone 11E10) (ABIN2192154) specifically detects Shiga Toxin 2 in IA, InhA 和 WB.
The antibody is reactive with Shigella dysenteriae samples.
(2 references)
产品编号 ABIN2192154
发货至:
中国
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Quick Overview for Shiga Toxin 2 (Stx-2) 抗体 (ABIN2192154)
抗原
Shiga Toxin 2 (Stx-2)
适用
Shigella dysenteriae
宿主
小鼠
克隆类型
单克隆
应用范围
Immunoassay (IA), Inhibition Assay (InhA), Western Blotting (WB)
克隆位点
11E10
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过滤
- 0.2 μm filtered
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应用备注
- For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For inhibition of biological activity in vitro dilutions have to be made according to the amounts of toxin to be inactivated.
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限制
- 仅限研究用
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缓冲液
- PBS, containing 0.1 % bovine serum albumin.
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储存条件
- 4 °C
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储存方法
- Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
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有效期
- 12 months
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: "Development of a hybrid Shiga holotoxoid vaccine to elicit heterologous protection against Shiga toxins types 1 and 2." in: Vaccine, Vol. 24, Issue 19, pp. 4122-9, (2006) (PubMed).
: "Isolation and characterization of monoclonal antibodies to Shiga-like toxin II of enterohemorrhagic Escherichia coli and use of the monoclonal antibodies in a colony enzyme-linked immunosorbent assay." in: Journal of clinical microbiology, Vol. 26, Issue 10, pp. 2127-31, (1988) (PubMed).
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- Shiga Toxin 2 (Stx-2)
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背景
- The monoclonal antibody 11E10 recognizes the 2A subunit of Shiga-like toxin 2. Shiga-like toxins (SLTs), are also called Verotoxins. Enterohemorrhagic Escherichia coli (EHEC) strains which are primarily of serotypes 0157:H7, 026:H11, and O111:H8 have been incriminated as etiologic agents of hemorrhagic colitis and Hemolytic-uremic syndrome, a generalized disease characterized by acute renal failure, thrombocytopenia, and microangiopathic hemolytic anemia. There are several distinct E.coli SLTs. SLT-I and SLT-II are produced by EHEC. SLT-I and Shiga toxin share >99 % deduced amino acid sequence homology, whereas SLT-I and SLT-II share about 60 % deduced amino acid sequence homology. SLT-I and SLT-II are antigenically distinct. Antibodies to SLT-II can also neutralize a variant of SLT-II (designated SLT-Iiv) produced by strains of E.coli that cause edema. SLT-IIv is cytotoxic for Vero but not HeLa cells, distinguishing it from SLT-II. The protein structure of the toxin consists of two domains: the A polypeptide that inhibits protein synthesis by targeting ribosomes, and the B polypeptide pentamer that binds to the eukaryotic cell receptor globotriaosylceramide (Gb3) leading to receptor-mediated endocytosis.
抗原
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