Cytotoxic Necrotizing Factor 1 抗体
适用: 化学剂
DB, WB, IA, InhA
宿主: 小鼠
Monoclonal
NG8
unconjugated
(2 references)-
- 抗原
- Cytotoxic Necrotizing Factor 1
- 适用
- 化学剂
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
- 应用范围
- Dot Blot (DB), Western Blotting (WB), Immunoassay (IA), Inhibition Assay (InhA)
- 过滤
- 0.2 μm filtered
- 克隆位点
- NG8
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- 应用备注
- For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For inhibition of biological activity in vitro dilutions have to be made according to the amounts of CNF1 to be inactivated.
- 限制
- 仅限研究用
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- 缓冲液
- PBS, containing 0.1 % bovine serum albumin.
- 储存条件
- 4 °C
- 储存方法
- Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
- 有效期
- 12 months
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: "Two domains of cytotoxic necrotizing factor type 1 bind the cellular receptor, laminin receptor precursor protein." in: Infection and immunity, Vol. 75, Issue 11, pp. 5095-104, (2007) (PubMed).
: "Epitope mapping of monoclonal antibodies capable of neutralizing cytotoxic necrotizing factor type 1 of uropathogenic Escherichia coli." in: Infection and immunity, Vol. 69, Issue 4, pp. 2066-74, (2001) (PubMed).
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: "Two domains of cytotoxic necrotizing factor type 1 bind the cellular receptor, laminin receptor precursor protein." in: Infection and immunity, Vol. 75, Issue 11, pp. 5095-104, (2007) (PubMed).
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- 抗原
- Cytotoxic Necrotizing Factor 1
- 物质类
- Chemical
- 背景
- The monoclonal antibody NG8 is specific for Cytotoxic necrotizing factor type 1 (CNF1) of uropathogenic Escherichia coli. CNF1 and CNF2 belong to a family of bacterial toxins that target the small GTP-binding Rho proteins that regulate the actin cytoskeleton. Members of this toxin family typically inactivate Rho, however, CNF1 and the highly related CNF2 activate Rho by deamidation. CNF1 is more frequently associated with E.coli strains that cause extraintestitinal infections in humans, particularly those of the urinary tract (such as cystitis, pyelonephritis and prostatitis). In CNF1-producing uropathogenic E. coli strains, CNF1 is chromosomally encoded and typically resides on a pathogenicity island that also contains hemolysin and P fimbria- related genes. Both CNF1 and the highly related, plasmid-encoded CNF2 are monomeric, cytoplasmic toxins of approximately 115 kDa. CNF1 can be structurally organized into three functional domains the N-terminal, central and the C-terminal domain. The latter exhibits the catalytic activity of the toxin. Monoclonal antibody NG8 recognizes an epitope between amino acids 704 and 730 of the C-terminal enzymatic domain. NG8 specifically neutralizes CNF1 while lacking activity for CNF2.
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