(2 references)-
- 抗原
- Lactoferricin B
- 适用
- Cow
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
- 应用范围
- Western Blotting (WB), Immunoassay (IA)
- 过滤
- 0.2 μm filtered
- 克隆位点
- 5F12-1-2
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- 应用备注
- For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. Please notice that the antibody detects a band of 3 kDa when western blot is used as application. This asks for a special SDS-Page or Tricine protocol.
- 限制
- 仅限研究用
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- 缓冲液
- PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
- 有效期
- 12 months
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: "Structural and immunochemical studies on bovine lactoferrin fragments." in: Advances in experimental medicine and biology, Vol. 443, pp. 41-8, (1998) (PubMed).
: "Monoclonal antibody against bovine Lactoferricin and its epitopic site." in: The Journal of veterinary medical science / the Japanese Society of Veterinary Science, Vol. 58, Issue 12, pp. 1227-9, (1997) (PubMed).
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: "Structural and immunochemical studies on bovine lactoferrin fragments." in: Advances in experimental medicine and biology, Vol. 443, pp. 41-8, (1998) (PubMed).
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- 抗原
- Lactoferricin B
- 别名
- Lf antibody, lactotransferrin antibody, LTF antibody
- 背景
- Monoclonal antibody clone 5F12.1.2, anti bovine Lactoferricin B is highly specific for bovine Lactoferricin B. This peptide is derived by enzymatic cleavage of lactoferrin which is a member of the transferrin family of metal-binding proteins found in milk and other secretory fluids and also in blood. Cleavage by pepsin of bovine lactoferrin leads to the release of Lactoferricin B (aminoacid 17-41). This peptide is highly basic, possessing five Arg (R) and three Lys (K) residues. In addition, a number of Trp (W) and Phe (F) aromatic residues are present. The two Cys (C) residues from lactoferricin B form a disulfide bond, generating an almost completely cyclical peptide. Nevertheless, the disulfide bond is not required for the antimicrobial potency. Several studies have shown that Lactoferricin B has a broad-spectrum activity against various Gram-positive and Gram-negative bacteria. In addition the peptide has been shown to have antifungal, antiviral and antitumour activity and to bind lipopolysaccharides (LPS, endotoxin). Moreover, it is known to stimulate the adaptive immune response and has anti-inflammatory properties. Lactoferricin B belongs to a large group of cationic antimicrobial peptides. The monoclonal antibody 5F12.1.2 is specific for bovine Lactoferricin B and detects the QWR antigenic determinant specific for bovine Lactoferricin B (3 kDa), it lacks reactivity with bovine lactoferrin C-lobe, human lactoferrin or lactoferricin H. The QWR sequence recognized by the antibody 5F12.1.2 is not present in lactoferrin in human, pig, mouse, goat, rabbit, horse, rat, cockroach and African clawed frog.
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