Endothelial Cells 抗体
This anti-Endothelial Cells antibody is a 小鼠 单克隆 antibody detecting Endothelial Cells in IHC (fro), IHC (p) 和 FACS. Suitable for 大鼠. This Primary Antibody has been cited in 5+ publications.
(5 references)Quick Overview for Endothelial Cells 抗体 (ABIN2192103)
抗原
Endothelial Cells
适用
大鼠
宿主
小鼠
克隆类型
单克隆
标记
This Endothelial Cells antibody is un-conjugated
应用范围
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)
克隆位点
RECA-1
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交叉反应 （详细）
- Cross reactivity: Goat : No, Human : No, Mouse : No, Rabbit : No, Sheep : No
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过滤
- 0.2 μm filtered
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亚型
- IgG1
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anti-Endothelial Cells antibody
适用: Pig IHC (fro), EIA 宿主: 小鼠 Monoclonal PE1 unconjugated
anti-Endothelial Cells antibody适用: 人 IHC (fro) 宿主: 大鼠 Monoclonal 1F10 unconjugated
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应用备注
- For immunohistology and flow cytometry dilutions to be used depends on tissue type and on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Positive Glomerular endothelial cells, RHEC cell line control Negative Vascular smooth muscle cells, fibroblasts control
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限制
- 仅限研究用
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缓冲液
- PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
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: "Blood-brain barrier abnormalities caused by exposure to HIV-1 gp120--protection by gene delivery of antioxidant enzymes." in: Neurobiology of disease, Vol. 38, Issue 2, pp. 313-25, (2010) (PubMed).
: "Heme-oxygenase upregulation ameliorates angiotensin II-induced tubulointerstitial injury and salt-sensitive hypertension." in: American journal of nephrology, Vol. 26, Issue 6, pp. 552-61, (2007) (PubMed).
: "Renal injury and salt-sensitive hypertension after exposure to catecholamines." in: Hypertension, Vol. 34, Issue 1, pp. 151-9, (1999) (PubMed).
: "Heart EC respond heterogeneous on cytokine stimulation in ICAM-1 and VCAM-1, but not in MHC expression. A study with 3 rat heart endothelial cell (RHEC) lines." in: Endothelium : journal of endothelial cell research, Vol. 5, Issue 4, pp. 307-19, (1998) (PubMed).
: "Antibodies defining rat endothelial cells: RECA-1, a pan-endothelial cell-specific monoclonal antibody." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 66, Issue 4, pp. 459-66, (1992) (PubMed).
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: "Blood-brain barrier abnormalities caused by exposure to HIV-1 gp120--protection by gene delivery of antioxidant enzymes." in: Neurobiology of disease, Vol. 38, Issue 2, pp. 313-25, (2010) (PubMed).
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- Endothelial Cells
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背景
- The monoclonal antibody RECA-1 reacts with Rat Endothelial Cell Antigen (RECA), a cell surface antigen (MCA970R) on rat endothelial cells. Endothelial cells (EC) line the interior of all blood vessels and are the key players in the angiogenesis cascade. EC are the first cells and barrier that vehicles or medicines encounter after systemic delivery. Furthermore, they have a signaling function to the cells of the immune system to indicate the status of the surrounding tissue. RECA-1is at least reactive with 1 n u the rat MHC-haplotype, Lewis (TR-1 ), BN (RT-1 ) and OA (RT-1 ). RECA-1 antibody has been successfully applied in staining of viable endothelial cells in vitro, and of vascular endothelium in vivo. No reactivity of the RECA-1 monoclonal antibody was seen with other cell types e.g. fibroblasts, leukocytes and non endothelial stromal cells nor with other various tested species other than rat e.g. mouse, rabbit, sheep, goat and human. RECA-1 is a promising antibody for rat endothelial cell studies, and in particular for further defining nature and function of endothelial cell-specific antigens.
抗原
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