(2 references)-
- 抗原
- Complement MASP3
- 适用
- 人
-
宿主
- 大鼠
-
克隆类型
- 单克隆
-
标记
- 非结合性
- 应用范围
- Western Blotting (WB), Immunoassay (IA)
- 过滤
- 0.2 μm filtered
- 克隆位点
- 38:12-3
-
anti-Complement MASP3 (AA 450-721) antibody
适用: 人 WB 宿主: 小鼠 Monoclonal MM0197-4F19 unconjugated
-
- 应用备注
- For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
- 限制
- 仅限研究用
-
- 缓冲液
- PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
- 有效期
- 12 months
-
-
: "Cooperation between MASP-1 and MASP-2 in the generation of C3 convertase through the MBL pathway." in: International immunology, Vol. 19, Issue 2, pp. 141-9, (2007) (PubMed).
: "Inherited deficiency of mannan-binding lectin-associated serine protease 2." in: The New England journal of medicine, Vol. 349, Issue 6, pp. 554-60, (2003) (PubMed).
-
: "Cooperation between MASP-1 and MASP-2 in the generation of C3 convertase through the MBL pathway." in: International immunology, Vol. 19, Issue 2, pp. 141-9, (2007) (PubMed).
-
- 抗原
- Complement MASP3
- 背景
- The monoclonal antibody 38:12-3 recognizes MASP-3, a 94 kDa mannan-binding lectin (MBL)- associated serine protease. Three pathways of complement activation have been reported: the antibody-dependent classical pathway, the antibody-independent alternative pathway and the lectin pathway. Activation of each pathway involves formation of serine protease complexes, which results in activation of the central complement component, C3. In the lectin pathway, mannose- binding-lectin (MBL)-associated serine proteases (MASP) form complexes with oligopolymeric lectin molecules, which are involved in pattern recognition. Upon binding of the recognition molecules to carbohydrates on the surface of micro- organisms, MASPs are converted to their active forms and initiate complement activation. Six members of the family of MASP-like serine proteases have been reported: MASP-1, MASP-2, MASP-3, Map19, C1r and C1s. These products show identical domain organization, even though the overall amino acid sequence identity is only approximately 40 % . Each MASP-type forms homodimers and is individually 2+ associated with MBL and the ficolins in a Ca -dependent matter. The four MBL-associated proteins are generated from only two genes, the MASP-1/3 gene encodes MASP-1 and MASP-3, whereas the MASP-2/Map19 gene gives rise to MASP-2 and the non-enzymatic MASP-2 MAp19. MASP-1 associates preferentially with low oligomer MBL (termed MBL-I), whereas MASP-2 and MASP-3 associate mainly with larger MBL oligomers (MBL II and MBL III). MASP-3 is generated by alternative splicing of the MASP-1/3 gene. The MASP-3 transcription product is composed of an A chain, which is common to both MASP-1 and MASP-3, and a B chain, which is unique to MASP- 3. The biological function of MASP-3 remains unclear. However, MASP-3 was found to downregulate the C4 and C2 cleaving activity of MASP-2. MASP-3 inhibits MASP-2 by a combination of competitive inhibition of MASP-2 association with MBL and inhibition of activation of the MBL-associated MASP-2. The monoclonal antibody 38:12-3 does not recognize MASP-1.
-
You are here:
