Mature Macrophages 抗体
This 小鼠 单克隆 antibody specifically detects Mature Macrophages in IHC (fro), FACS, IHC (p) 和 WB. It exhibits reactivity toward 人. It has been mentioned in 3+ publications
(3 references)
产品编号 ABIN2192020
发货至:
中国
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Contact Info
Our Local Distributor
中国
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Quick Overview for Mature Macrophages 抗体 (ABIN2192020)
抗原
Mature Macrophages
适用
人
宿主
小鼠
克隆类型
单克隆
应用范围
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
克隆位点
25F9
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过滤
- 0.2 μm filtered
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应用备注
- For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
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限制
- 仅限研究用
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缓冲液
- PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
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有效期
- 12 months
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: "Phenotypic characterization of alveolar monocyte recruitment in acute respiratory distress syndrome." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 279, Issue 1, pp. L25-35, (2000) (PubMed).
: "Macrophage differentiation antigens in acute and chronic autoimmune polyneuropathies." in: Brain : a journal of neurology, Vol. 121 ( Pt 3), pp. 469-79, (1998) (PubMed).
: "A monoclonal antibody to a differentiation antigen present on mature human macrophages and absent from monocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 134, Issue 3, pp. 1487-92, (1985) (PubMed).
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- Mature Macrophages
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背景
- The monoclonal antibody 25F9 recognises a protein of 86 kD on the cell surface and within the cytoplasm of mature macrophages. The antibody is associated with fully differentiated tissue macrophages both in normal and in diseased tissues, independently of the presence or absence of inflammation. The antigen is absent on freshly isolated monocytes and other blood cells. After 6 to 7 days culture human monocytes become positive. Some melanoma and carcinoma cell lines are also positive. Furthermore the monoclonal antibody 25F9 cross reacts with a subpopulation of macrophages of rhesus monkey, pig alveolar macrophages and Kupffer cells. The monoclonal antibody 25F9 is very useful for macrophage phenotyping.
抗原
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