(1 reference)-
- 抗原 See all FHL2 抗体
- FHL2 (Four and A Half LIM Domains 2 (FHL2))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This FHL2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 过滤
- 0.2 μm filtered
- 克隆位点
- F4B2-B11
- Top Product
- Discover our top product FHL2 Primary Antibody
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anti-Four and A Half LIM Domains 2 (FHL2) (AA 1-279) antibody
FHL2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Four and A Half LIM Domains 2 (FHL2) antibodyFHL2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Four and A Half LIM Domains 2 (FHL2) (AA 1-279) antibodyFHL2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2G3-1A5 unconjugated
anti-Four and A Half LIM Domains 2 (FHL2) (AA 112-233) antibodyFHL2 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Four and A Half LIM Domains 2 (FHL2) (AA 199-248) antibodyFHL2 适用: 人, 小鼠, 大鼠, 犬, 马, 斑马鱼, 猴, Bat, Hamster, 小鸡 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Four and A Half LIM Domains 2 (FHL2) antibodyFHL2 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-Four and A Half LIM Domains 2 (FHL2) (C-Term) antibodyFHL2 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 豚鼠, 兔, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Four and A Half LIM Domains 2 (FHL2) antibodyFHL2 适用: 人, 小鼠 WB, IHC (p), IP 宿主: 小鼠 Monoclonal 31-10-34 unconjugated
anti-Four and A Half LIM Domains 2 (FHL2) (C-Term) antibodyVerified FHL2 适用: 人, 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Four and A Half LIM Domains 2 (FHL2) (AA 182-231) antibodyFHL2 适用: 人, Cow, 豚鼠, 猴 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- For immunohistology and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
- 限制
- 仅限研究用
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- 缓冲液
- PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
- 有效期
- 12 months
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: "The LIM-only protein FHL2 interacts with beta-catenin and promotes differentiation of mouse myoblasts." in: The Journal of cell biology, Vol. 159, Issue 1, pp. 113-22, (2002) (PubMed).
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: "The LIM-only protein FHL2 interacts with beta-catenin and promotes differentiation of mouse myoblasts." in: The Journal of cell biology, Vol. 159, Issue 1, pp. 113-22, (2002) (PubMed).
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- 抗原
- FHL2 (Four and A Half LIM Domains 2 (FHL2))
- 别名
- Fhl2 (FHL2 产品)
- 别名
- AAG11 antibody, DRAL antibody, FHL-2 antibody, SLIM-3 antibody, SLIM3 antibody, C76204 antibody, FHL2 antibody, zgc:92428 antibody, zgc:103466 antibody, four and a half LIM domains 2 antibody, four and a half LIM domains 2a antibody, four and a half LIM domains 2b antibody, FHL2 antibody, Fhl2 antibody, fhl2 antibody, fhl2a antibody, fhl2b antibody
- 背景
- The monoclonal antibody F4B2-B11 reacts specifically with the LIM-only protein FHL2. Five proteins share the same structural organization and a high degree of sequence homology in this group: four and a half LIM domain protein (FHL) 1, FHL2, FHL3, FHL4, and activator of cAMP-responsive element (CRE) modulator (CREM) in testis (ACT). LIM domains are constituted by a conserved cysteine- and histidine- rich structure shaped in two repeated zinc fingers first identified in the proteins encoded by the Lin-11, Isl- 1, and Mec-3 genes. The LIM domain has been shown to function as a protein-protein interaction domain, and has often been described in association with other functional protein motifs, such as homeobox and kinase domains. FHL2 seems to be a promiscuous coactivator because it modulates the activity of the androgen receptor, CRE-binding protein (CREB), and WT1, although some degree of specificity is present because it is unable to stimulate CREM- and Sp1-dependent transcription. FHL2 expression was originally described to be restricted to the heart but it is inducible in other cell types. FHL2 shows specific interaction with beta-catenin, which requires the intact structure of all four LIM domains of FHL2 and the N-terminus plus the first armadillo repeat region of beta-catenin. FHL2 is a muscle-specific repressor of LEF/TCF target genes and promotes myogenic differentiation by interacting with beta-catenin. Monoclonal antibody F4B2-B11 recognizes the N-terminal Zn-Finger motif, it does not crossreact in Western Blotting with the FHL1 and FHL3 proteins. The F4B2-B11 antibody is cross reactive with mouse and rat FHL2.
- 途径
- Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha
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