Activated c1s 抗体
适用: 人
IP, IHC (p), WB, IA, IHC (fro), FACS, InhA
宿主: 小鼠
Monoclonal
M241
unconjugated
(1 reference)-
- 抗原
- Activated c1s
- 适用
- 人
- 宿主
- 小鼠
- 克隆类型
- 单克隆
- 应用范围
- Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunoassay (IA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Inhibition Assay (InhA)
- 过滤
- 0.2 μm filtered
- 克隆位点
- M241
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- 应用备注
- For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For inhibition of biological activity dilutions have to be made according to the amounts C1s to be inactivated.
- 限制
- 仅限研究用
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- 缓冲液
- PBS containing 0.1 % bovine serum albumin.
- 储存条件
- 4 °C
- 储存方法
- Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
- 有效期
- 12 months
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: "Probing the C4-binding site on C1s with monoclonal antibodies. Evidence for a C4/C4b-binding site on the gamma-domain." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 8, pp. 2743-50, (1989) (PubMed).
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: "Probing the C4-binding site on C1s with monoclonal antibodies. Evidence for a C4/C4b-binding site on the gamma-domain." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 8, pp. 2743-50, (1989) (PubMed).
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- 抗原
- Activated c1s
- 背景
- Monoclonal antibody M241 reacts with an epitope on human C protein activated C1s, a subcomponent of the first component of C (C1). Activated C1s is a glycosylated single-polypeptide zymogen of 85 kD. Activation of the proenzyme C1s occurs through cleavage by the active form of C1r. The activated protease, activated C1s, consists of a disulfide-linked H chain and a L chain. Activated C1s is a serine protease and its catalytic site is located in the L chain. Activation of the classical C pathway is triggered by activated C1s which cleaves C4 and C2 to form the C3 convertase, C4bC2a. The epitope recognised by the antibody M241 is domain IV and/or V of the gamma-domain of activated C1s. Monoclonal antibody M241 blocks C4 activation and C4 binding to activated C1s. The antibody binds specifically to the active centre of C1s.
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