M-Csf Responsive Cells 抗体
适用: 小鼠
IHC (fro), FACS
宿主: 大鼠
Monoclonal
ER-MP58
unconjugated
(4 references)-
- 抗原
- M-Csf Responsive Cells
- 适用
- 小鼠
- 宿主
- 大鼠
- 克隆类型
- 单克隆
- 应用范围
- Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
- 过滤
- 0.2 μm filtered
- 克隆位点
- ER-MP58
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- 应用备注
- For immunohistology and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
- 限制
- 仅限研究用
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- 缓冲液
- PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
- 有效期
- 12 months
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: "The effect of tissue type-plasminogen activator deletion and associated fibrin(ogen) deposition on macrophage localization in peritoneal inflammation." in: Thrombosis and haemostasis, Vol. 95, Issue 4, pp. 659-67, (2006) (PubMed).
: "Subpopulations of mouse blood monocytes differ in maturation stage and inflammatory response." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 7, pp. 4410-7, (2004) (PubMed).
: "Macrophage lineage cells in inflammation: characterization by colony-stimulating factor-1 (CSF-1) receptor (c-Fms), ER-MP58, and ER-MP20 (Ly-6C) expression." in: Blood, Vol. 92, Issue 4, pp. 1423-31, (1998) (PubMed).
: "High-level expression of the ER-MP58 antigen on mouse bone marrow hematopoietic progenitor cells marks commitment to the myeloid lineage." in: European journal of immunology, Vol. 26, Issue 12, pp. 2850-8, (1997) (PubMed).
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: "The effect of tissue type-plasminogen activator deletion and associated fibrin(ogen) deposition on macrophage localization in peritoneal inflammation." in: Thrombosis and haemostasis, Vol. 95, Issue 4, pp. 659-67, (2006) (PubMed).
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- 抗原
- M-Csf Responsive Cells
- 背景
- The monoclonal antibody ER-MP58 recognizes an antigen present on all mouse macrophage colony- stimulating factor (M-CSF) responsive cells in the bone marrow, including the earliest colony-forming myeloid progenitors, as well as by the majority of other myeloid precursors. The antigen is detected on a broad development range of macrophage precursor cells to the monocytic level, but also on granulocytes. Expression is rapidly lost upon maturation beyond the monocytic stage. The antigen disappears in the course of macrophage differentiation. Furthermore the antigen is clearly different from commonly used myeloid markers as Mac-1, F4/80, and Gr-1. The monoclonal antibody ER-MP58 is very useful for the identification of mouse myeloid hematopoietic islands in various organs, and for embryonic studies. Cells committed to the myeloid lineage can be separated from progenitor cells with other differentiation capacities by means of multiparameter cell sorting using monoclonal antibody ER-MP58 in combination with monoclonal antibody ER-MP12 (HM1084) and ER-MP-20 (HM1082).
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