Ly6C 抗体
(4 references)-
- 抗原 See all Ly6C (Ly-6C) products
- Ly6C (Ly-6C) (Ly-6C)
- 适用
- 小鼠
-
宿主
- 大鼠
-
克隆类型
- 单克隆
-
标记
- This Ly6C antibody is un-conjugated
-
应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoassay (IA), Detection (D)
- 过滤
- 0.2 μm filtered
- 克隆位点
- ER-MP20
-
anti-Ly-6C (Ly-6C) antibody
Ly-6C 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal HK1-4 unconjugated
anti-Ly-6C (Ly-6C) antibodyLy-6C 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Ly-6C (Ly-6C) antibodyLy-6C 适用: 小鼠 FACS, IHC (fro) 宿主: 大鼠 Monoclonal HK1-4 unconjugated
anti-Ly-6C (Ly-6C) antibody (PE)Ly-6C 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal HK1-4 PE
anti-Ly-6C (Ly-6C) antibody (Biotin)Ly-6C 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal HK1-4 Biotin
anti-Ly-6C (Ly-6C) antibody (FITC)Ly-6C 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal HK1-4 FITC
-
- 应用备注
- For immunohistology and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
- 限制
- 仅限研究用
-
- 缓冲液
- PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
- 有效期
- 12 months
-
-
: "Loss of S100A9 (MRP14) results in reduced interleukin-8-induced CD11b surface expression, a polarized microfilament system, and diminished responsiveness to chemoattractants in vitro." in: Molecular and cellular biology, Vol. 23, Issue 3, pp. 1034-43, (2003) (PubMed).
: "Commitment to the monocytic lineage occurs in the absence of the transcription factor PU.1." in: Blood, Vol. 93, Issue 9, pp. 2849-58, (1999) (PubMed).
: "Macrophage lineage cells in inflammation: characterization by colony-stimulating factor-1 (CSF-1) receptor (c-Fms), ER-MP58, and ER-MP20 (Ly-6C) expression." in: Blood, Vol. 92, Issue 4, pp. 1423-31, (1998) (PubMed).
: "Distinct mouse bone marrow macrophage precursors identified by differential expression of ER-MP12 and ER-MP20 antigens." in: European journal of immunology, Vol. 24, Issue 10, pp. 2279-84, (1994) (PubMed).
-
: "Loss of S100A9 (MRP14) results in reduced interleukin-8-induced CD11b surface expression, a polarized microfilament system, and diminished responsiveness to chemoattractants in vitro." in: Molecular and cellular biology, Vol. 23, Issue 3, pp. 1034-43, (2003) (PubMed).
-
- 抗原
- Ly6C (Ly-6C) (Ly-6C)
- Abstract
- Ly-6C 产品
- 别名
- AA682074 antibody, AA959465 antibody, Ly-6C antibody, Ly-6C1 antibody, Ly6c antibody, lymphocyte antigen 6 complex, locus C1 antibody, Ly6c1 antibody
- 背景
- The monoclonal antibody ER-MP20 specifically reacts with mouse macrophage precursor cells in the mid-stage of their development (late CFU-M, monoblasts and monocytes). The antigen is a 14 kD surface protein which is very similar to Ly-6C and may be analogous to human CD59. It is inducible by IFN-alpha, IFN-beta and IFN-gamma. In tissue sections, the antigen is found on macrophage precursor subpopulations. In the bone marrow and hemopoietic islands of the lymphoid organs, and in the spleen. Activated macrophages in inflammatory tissues also express the ER-MP20-related antigen. The monoclonal antibody ER-MP20 has been raised after immunization of rats with mouse macrophage cell lines and reacts with mouse macrophage precursor cells. The monoclonal antibody also identifies activated macrophages in inflammatory tissues where the simultaneous use of the murine pan- macrophage marker BM8 (anti-F4/80) is recommended. In combination with an anti-mouse CD31/PECAM-1 antibody, ER-MP20 can be used to evaluate the cellular composition in murine bone marrow (e.g. using flow cytometric analysis). ER-MP20 also detects a wide range of endothelial cells.
-
