(1 reference)-
- 抗原
- Radioprotective 105
- 适用
- 小鼠
- 宿主
- 大鼠
- 克隆类型
- 单克隆
- 应用范围
- Activation (Act), Functional Studies (Func), Flow Cytometry (FACS)
- 过滤
- 0.2 μm filtered
- 克隆位点
- RP-14
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- 应用备注
- For flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.
- 限制
- 仅限研究用
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- 缓冲液
- PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
- 有效期
- 12 months
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: "Requirement for MD-1 in cell surface expression of RP105/CD180 and B-cell responsiveness to lipopolysaccharide." in: Blood, Vol. 99, Issue 5, pp. 1699-705, (2002) (PubMed).
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: "Requirement for MD-1 in cell surface expression of RP105/CD180 and B-cell responsiveness to lipopolysaccharide." in: Blood, Vol. 99, Issue 5, pp. 1699-705, (2002) (PubMed).
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- 抗原
- Radioprotective 105
- 背景
- Radioprotective 105 (RP105, CD180) is a type 1 transmembrane protein of 105 kDa with extracellular leucine-riche repeats (LLRs) and a short cytoplasmic tail. RP105, assigned as CD180, is similar to Drosophila Toll in the extracellular LLRs. Radioprotective 105 (RP105, CD180) expression is largely + restricted to immune cells including CD19 B cells and macrophages. Histological studies showed that RP105 is expressed mainly on mature B cells in mantle zones. Antibody-mediated (RP/14) cross-linking of RP105 induces resistance against irradiation-induced apoptosis, B-cell proliferation, and up-regulation of a costimulatory molecule B7.2, revealing RP105 as a potent regulator of B-cell activation. RP105 (CD180) has an important role in B-cell activation by LPS. It is important to note that RP105 associates with MD1, which is in this context important for RP105 with respect to B-cell surface expression and LPS recognition and signalling. MD-1 seems to be requisite for efficient expression of RP105.
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