IL-5 抗体
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- 抗原 See all IL-5 (IL5) 抗体
- IL-5 (IL5) (Interleukin 5 (IL5))
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适用
- 人, 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This IL-5 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Neutralization (Neut), Immunofluorescence (IF)
- 特异性
- Recognizes mouse and human Interleukin-5 (IL-5).
- 纯化方法
- Purified
- 免疫原
- Semi-purified mouse T cell clone supernatant.
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product IL5 Primary Antibody
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- 应用备注
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Approved: ELISA, Flo, IF, Neut, WB
Usage: ELISA Capture: Useful as a capture antibody in a sandwich ELISA for quantifying mouse or human IL-5 protein levels.5-7 Purified antibody should be paired with biotinylated antibody (Cat. Immunofluorescence/. Flow Cytometry: Useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-5-positive cells within mixed cell populations.8 Recombinant mouse IL-5 or Rat IgG1 should be used as a specificity control. Western Blot: The purified antibody is also useful for Western blot applications. For Western blotting, a concentration of 1-5 μg/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant mouse IL-5 under reducing conditions. Neutralization: The LE/AF form can be used for neutralizing mouse IL-5 bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. - 说明
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Target Species of Antibody: Mouse
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS. No preservative added.
- 储存液
- Azide free
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
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- 抗原
- IL-5 (IL5) (Interleukin 5 (IL5))
- 别名
- IL5 (IL5 产品)
- 背景
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Name/Gene ID: IL5
Family: Hormone
Synonyms: IL5, EDF, Interleukin-5, IL-5, T-cell replacing factor, TRF - 基因ID
- 3567
- UniProt
- P05113
- 途径
- JAK/STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
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