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IL-5 抗体 (Biotin)

This anti-IL-5 antibody is a 大鼠 单克隆 antibody detecting IL-5 in WB, ELISA, FACS 和 IF. Suitable for 人.
产品编号 ABIN218119
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for IL-5 抗体 (Biotin) (ABIN218119)

抗原

See all IL-5 (IL5) 抗体
IL-5 (IL5) (Interleukin 5 (IL5))

适用

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宿主

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大鼠

克隆类型

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单克隆

标记

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This IL-5 antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)
  • 特异性

    Recognizes human Interleukin-5 (IL-5).

    纯化方法

    Purified

    免疫原

    COS-expressed recombinant human IL-5.

    亚型

    IgG2a kappa
  • 应用备注

    Approved: ELISA, Flo, IF, WB

    Usage: ELISA: Monoclonal antibody is useful as a capture or detection antibody in a sandwich ELISA for quantifying human IL-5 protein levels.1-3,5 Purified antibody may be paired with biotinylated antibody as the detection antibody, with purified recombinant human IL-5 as the standard. Or, conversely, biotinylated may be paired with purified antibody as the capture antibody. Immunofluorescence/Flow Cytometry: This antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-5-positive cells within mixed cell populations.5,6 Recombinant human IL-5, unlabeled antibody, or Rat IgG1 should be used as a specificity control. Western Blot: The purified antibody is also useful for Western blotting applications. A concentration of 1-5 μg/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant human IL-5 under reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS, sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C
    Long term: Add glycerol (40-50%) -20°C.
  • 抗原

    IL-5 (IL5) (Interleukin 5 (IL5))

    别名

    IL5

    背景

    Name/Gene ID: IL5
    Family: Hormone

    Synonyms: IL5, EDF, Interleukin-5, IL-5, T-cell replacing factor, TRF

    基因ID

    3567

    UniProt

    P05113

    途径

    JAK/STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
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