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IL-5 抗体 (Biotin)

IL5 适用: 人 WB, ELISA, FACS, IF 宿主: 大鼠 Monoclonal Biotin
产品编号 ABIN218119
发货至: 中国
  • 抗原 See all IL-5 (IL5) 抗体
    IL-5 (IL5) (Interleukin 5 (IL5))
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    大鼠
    克隆类型
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    单克隆
    标记
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    This IL-5 antibody is conjugated to Biotin
    应用范围
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    Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)
    特异性
    Recognizes human Interleukin-5 (IL-5).
    纯化方法
    Purified
    免疫原
    COS-expressed recombinant human IL-5.
    亚型
    IgG2a kappa
    Top Product
    Discover our top product IL5 Primary Antibody
  • 应用备注
    Approved: ELISA, Flo, IF, WB

    Usage: ELISA: Monoclonal antibody is useful as a capture or detection antibody in a sandwich ELISA for quantifying human IL-5 protein levels.1-3,5 Purified antibody may be paired with biotinylated antibody as the detection antibody, with purified recombinant human IL-5 as the standard. Or, conversely, biotinylated may be paired with purified antibody as the capture antibody. Immunofluorescence/Flow Cytometry: This antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-5-positive cells within mixed cell populations.5,6 Recombinant human IL-5, unlabeled antibody, or Rat IgG1 should be used as a specificity control. Western Blot: The purified antibody is also useful for Western blotting applications. A concentration of 1-5 μg/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant human IL-5 under reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    PBS, sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Short term: 4°C
    Long term: Add glycerol (40-50%) -20°C.
  • 抗原
    IL-5 (IL5) (Interleukin 5 (IL5))
    别名
    IL5 (IL5 产品)
    背景
    Name/Gene ID: IL5
    Family: Hormone

    Synonyms: IL5, EDF, Interleukin-5, IL-5, T-cell replacing factor, TRF
    基因ID
    3567
    UniProt
    P05113
    途径
    JAK/STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
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