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IL-4 抗体 (Biotin)

This Biotin-conjugated 大鼠 单克隆 anti-IL-4 antibody specifically detects IL-4 in WB, ELISA, FACS 和 IF. The antibody is reactive with 人 samples.
产品编号 ABIN218108
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for IL-4 抗体 (Biotin) (ABIN218108)

抗原

See all IL-4 (IL4) 抗体
IL-4 (IL4) (Interleukin 4 (IL4))

适用

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宿主

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大鼠

克隆类型

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单克隆

标记

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This IL-4 antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)
  • 特异性

    Recognizes human Interleukin-4 (IL-4). Has been reported to cross-react with IL-4 from Rhesus monkeys. Has also been reported to be useful for epitope mapping of human IL-4.

    纯化方法

    Purified

    免疫原

    CHO-expressed recombinant human IL-4.

    亚型

    IgG1 kappa
  • 应用备注

    Approved: ELISA, Flo, IF, WB

    Usage: ELISA Detection: Monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying human IL-4 protein levels.1,3 Biotinylated antibody should be paired with a suitable purified antibody as the capture antibody, with purified recombinant human IL-4 as the standard. Immunofluorescence/. Flow Cytometry: This antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-4-positive cells within mixed cell populations. Recombinant human IL-4, unlabeled antibody, or Biotinylated Rat IgG1 should be used as a specificity control. Western Blot: The purified antibody is also useful for Western blotting applications. A concentration of 1-5 μg/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant human IL-4 under reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS, sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C
    Long term: Add glycerol (40-50%) -20°C.
  • 抗原

    IL-4 (IL4) (Interleukin 4 (IL4))

    别名

    IL4

    背景

    Name/Gene ID: IL4
    Family: Hormone

    Synonyms: IL4, B cell growth factor 1, B-cell stimulatory factor 1, B_cell stimulatory factor 1, BCGF1, BSF-1, Binetrakin, BCGF-1, Interleukin 4, Interleukin-4, Pitrakinra, BSF1, IL-4

    基因ID

    3565

    UniProt

    P05112

    途径

    JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
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