IL-4 抗体 (Biotin)
-
- 抗原 See all IL-4 (IL4) 抗体
- IL-4 (IL4) (Interleukin 4 (IL4))
-
适用
- 人
-
宿主
- 大鼠
-
克隆类型
- 单克隆
-
标记
- This IL-4 antibody is conjugated to Biotin
-
应用范围
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)
- 特异性
- Recognizes human Interleukin-4 (IL-4). Has been reported to cross-react with IL-4 from Rhesus monkeys. Has also been reported to be useful for epitope mapping of human IL-4.
- 纯化方法
- Purified
- 免疫原
- CHO-expressed recombinant human IL-4.
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product IL4 Primary Antibody
-
-
- 应用备注
-
Approved: ELISA, Flo, IF, WB
Usage: ELISA Detection: Monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying human IL-4 protein levels.1,3 Biotinylated antibody should be paired with a suitable purified antibody as the capture antibody, with purified recombinant human IL-4 as the standard. Immunofluorescence/. Flow Cytometry: This antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-4-positive cells within mixed cell populations. Recombinant human IL-4, unlabeled antibody, or Biotinylated Rat IgG1 should be used as a specificity control. Western Blot: The purified antibody is also useful for Western blotting applications. A concentration of 1-5 μg/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant human IL-4 under reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - 说明
-
Target Species of Antibody: Human
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
-
Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
-
- 抗原
- IL-4 (IL4) (Interleukin 4 (IL4))
- 别名
- IL4 (IL4 产品)
- 背景
-
Name/Gene ID: IL4
Family: Hormone
Synonyms: IL4, B cell growth factor 1, B-cell stimulatory factor 1, B_cell stimulatory factor 1, BCGF1, BSF-1, Binetrakin, BCGF-1, Interleukin 4, Interleukin-4, Pitrakinra, BSF1, IL-4 - 基因ID
- 3565
- UniProt
- P05112
- 途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
-