IL-4 抗体 (Biotin)
Quick Overview for IL-4 抗体 (Biotin) (ABIN218108)
抗原
See all IL-4 (IL4) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes human Interleukin-4 (IL-4). Has been reported to cross-react with IL-4 from Rhesus monkeys. Has also been reported to be useful for epitope mapping of human IL-4.
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纯化方法
- Purified
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免疫原
- CHO-expressed recombinant human IL-4.
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亚型
- IgG1 kappa
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应用备注
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Approved: ELISA, Flo, IF, WB
Usage: ELISA Detection: Monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying human IL-4 protein levels.1,3 Biotinylated antibody should be paired with a suitable purified antibody as the capture antibody, with purified recombinant human IL-4 as the standard. Immunofluorescence/. Flow Cytometry: This antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-4-positive cells within mixed cell populations. Recombinant human IL-4, unlabeled antibody, or Biotinylated Rat IgG1 should be used as a specificity control. Western Blot: The purified antibody is also useful for Western blotting applications. A concentration of 1-5 μg/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant human IL-4 under reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
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- IL-4 (IL4) (Interleukin 4 (IL4))
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别名
- IL4
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背景
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Name/Gene ID: IL4
Family: Hormone
Synonyms: IL4, B cell growth factor 1, B-cell stimulatory factor 1, B_cell stimulatory factor 1, BCGF1, BSF-1, Binetrakin, BCGF-1, Interleukin 4, Interleukin-4, Pitrakinra, BSF1, IL-4 -
基因ID
- 3565
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UniProt
- P05112
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
抗原
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