我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

Nanog 抗体 (AA 101-200)

Name: Nanog 抗体 (AA 101-200)
SKU: ABIN2177828
Availability: OutOfStock

NANOG 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, ICC, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Formalin-fixed and paraffin embedded rat testis labeled with Rabbit Anti-Nanog Polyclonal Antibodyy, Unconjugated (ABIN2177828) at 1:200 followed by conjugation to the secondary antibody and DAB staining (Nanog 抗体 (AA 101-200))

IHC (p)

NIH/3T3 lysates probed with Nanog Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation. (Nanog 抗体 (AA 101-200))

A431 lysates probed with Nanog Polyclonal Antibody, Unconjugated at 1:300 dilution and 4˚C overnight incubation. (Nanog 抗体 (AA 101-200))

Formalin-fixed and paraffin embedded rat embryo labeled with Rabbit Anti-Nanog Polyclonal Antibodyy, Unconjugated (ABIN2177828) at 1:200 followed by conjugation to the secondary antibody and DAB staining (Nanog 抗体 (AA 101-200))

IHC (p)

Formalin-fixed and paraffin embedded rat testis labeled with Anti-Nanog Polyclonal Antibody, Unconjugated (ABIN2177828) at 1:200 followed by conjugation to the secondary antibody and DAB staining (Nanog 抗体 (AA 101-200))

IHC (p)

5 images

(1 reference)

产品编号 ABIN2177828

发货至: 中国

Datasheet as PDF

抗原 See all Nanog (NANOG) 抗体

Nanog (NANOG) (Nanog Homeobox (NANOG))
抗原表位
15

14

10

9

8

7

7

5

5

4

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 101-200
适用
127

52

20

3

3

2

2

2

2

2

2

1

1
人, 小鼠, 大鼠
宿主
118

17

3
兔
Compatible Secondaries
克隆类型
108

30
多克隆
标记
74

9

7

7

5

4

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2
This Nanog antibody is un-conjugated
应用范围
101

62

47

40

28

23

23

19

15

11

10

7

2

1

1
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

人, 小鼠, 大鼠

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from mouse Nanog

亚型

IgG

Top Product

Discover our top product NANOG Primary Antibody
anti-Nanog Homeobox (NANOG) antibody
NANOG 适用: 人 WB, ELISA, IF, IHC, ICC, IHC (p), IP, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 1-305) antibody
NANOG 适用: 人 WB, ELISA, IF, FACS, ICC 宿主: 小鼠 Monoclonal 8A1D11 unconjugated

anti-Nanog Homeobox (NANOG) (AA 71-170) antibody
NANOG 适用: 人, 小鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 15-49) antibody
NANOG 适用: 人 WB, ELISA, IF, IHC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 98-195) antibody
NANOG 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2C11 unconjugated

anti-Nanog Homeobox (NANOG) (AA 98-195) antibody
NANOG 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3A12 unconjugated

anti-Nanog Homeobox (NANOG) (Internal Region) antibody
Verified NANOG 适用: 人 WB, ELISA, IF, IHC 宿主: 山羊 Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 20-166) antibody
NANOG 适用: 人 WB, ELISA, ICC 宿主: 小鼠 Monoclonal 1E6C4 unconjugated

anti-Nanog Homeobox (NANOG) (AA 267-292) antibody
NANOG 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 98-195) antibody
NANOG 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2E11 unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Nasr, Farghaly, Elsaba, El-Mokhtar, Radwan, Elsabahy, Abdelkareem, Fakhry, Mousa: "Resistance of primary breast cancer cells with enhanced pluripotency and stem cell activity to sex hormonal stimulation and suppression." in: The international journal of biochemistry & cell biology, Vol. 105, pp. 84-93, (2019) (PubMed).
抗原

Nanog (NANOG) (Nanog Homeobox (NANOG))

别名

Nanog (NANOG 产品)

背景

Synonyms: ENK, ecat4, 2410002E02Rik, Homeobox protein NANOG, ES cell-associated protein 4, Early embryo specific expression NK-type homeobox protein, Homeobox transcription factor Nanog, Nanog
Background: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

基因ID

71950

UniProt

Q80Z64

途径

Stem Cell Maintenance