Desmocollin 1 抗体 (Intracellular)
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- 抗原 See all Desmocollin 1 (DSC1) 抗体
- Desmocollin 1 (DSC1)
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抗原表位
- Intracellular
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Desmocollin 1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- Antigen recognized in species specific for human. Cell Lines (tested so far) of differentiated cells. All simple epithelia are negative. desmocollin 1, negative with bovine (tested so far) and rodent material. Reacts with 111kD (form 'a') and 103kD (form 'b') polypeptides (desmocollin1 splice forms, both at pI 5.2) from human epidermal desmosomes. Reactivities on Cultured Positive on primary keratinocytes, however, restricted to local piles.
- 预测反应
- Percent identity by BLAST analysis: Human, Gibbon, Monkey (96%) Gorilla (92%) Marmoset (88%).
- 纯化方法
- Protein A purified
- 免疫原
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Synthetic peptide corresponding to sequence present within the intracellular part of human desmocollin 1 (HQTLESVKGVGQGDTGRYATDWQC). Percent identity by BLAST analysis: Human, Gibbon, Monkey (96%), Gorilla (92%), Marmoset (88%).
Type of Immunogen: Synthetic peptide - 亚型
- IgG1
- Top Product
- Discover our top product DSC1 Primary Antibody
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anti-Desmocollin 1 (DSC1) (AA 486-686) antibody
DSC1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Desmocollin 1 (DSC1) (AA 480-690) antibodyDSC1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Desmocollin 1 (DSC1) antibodyDSC1 适用: 人, 小鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Desmocollin 1 (DSC1) antibodyDSC1 适用: 人 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Desmocollin 1 (DSC1) (AA 30-353) antibodyDSC1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Desmocollin 1 (DSC1) (AA 135-340) antibodyDSC1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Desmocollin 1 (DSC1) (AA 480-690) antibodyDSC1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Desmocollin 1 (DSC1) (AA 659-687), (C-Term) antibodyDSC1 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB21405 unconjugated
anti-Desmocollin 1 (DSC1) (AA 659-687), (C-Term) antibodyDSC1 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Approved: IHC, IHC-Fr, IHC-P, WB
Usage: Suitable for use in Immunohistochemistry and Western Blot. Immunohistochemistry (Frozen, Paraffin, HEIR): 1:10 in PBS, pH 7.4, Incubation Time 1 hr at RT or overnight at 4?C. Note: For better resolution on frozen sections preincubation (directly after fixation) with 0.05-0.2 % Triton X-100, for 5-10 min, depending on tissue type, is recommended (see ref. 2). Without detergent pretreatment a cytoplasmic component might cross-react (e. g. in fibroblasts). (Paraffin: slight cross-reactivity with a lymphocyte subpopulation is abolished by mild trypsinization after the microwave step. Localization: Localizes Dsc1 present in suprabasal layers of interfollicular epidermis, specific cell layers (e. g. in hair follicle root sheath), Hassall bodies (spheroidal formations of densely packed reticulum-derived cells in the thymus). - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Restore with 1 mL sterile ddH2O
- 浓度
- Lot specific
- 缓冲液
- Lyophilized powder from PBS, pH 7.4, 0.5 % BSA, 0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 1 year at -20°C.
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- 抗原
- Desmocollin 1 (DSC1)
- 别名
- DSC1 / Desmocollin 1 (DSC1 产品)
- 别名
- DSC1 antibody, CDHF1 antibody, DG2/DG3 antibody, DSC1b antibody, DG2DG3 antibody, dg3 antibody, Dsc1a antibody, Dsc1b antibody, desmocollin 1 antibody, DSC1 antibody, Dsc1 antibody
- 背景
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Name/Gene ID: DSC1
Family: Cadherin
Synonyms: DSC1, Desmocollin-1, Desmocollin 1, Desmosomal glycoprotein 2/3, DG2/DG3, Cadherin family member 1, CDHF1 - 基因ID
- 1823
- UniProt
- Q08554
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