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CDK1 抗体

The 小鼠 单克隆 anti-CDK1 antibody is suitable to detect CDK1 in samples from 人, 小鼠, 大鼠, 小鸡 和 非洲爪蟾. It has been validated for WB, IHC, IF, IP, IHC (p), IHC (fro) 和 Func.
产品编号 ABIN215066
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for CDK1 抗体 (ABIN215066)

抗原

See all CDK1 抗体
CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

适用

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人, 小鼠, 大鼠, 小鸡, 非洲爪蟾

宿主

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小鼠

克隆类型

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单克隆

标记

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This CDK1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)
  • 特异性

    Recognizes a 34kD protein cdk1, identified as cdk1/p34cdc2 protein kinase (a catalytic subunit of Maturation Promoting Factor). Its epitope maps near the C-terminus of protein and its core is thought to be LGTPNNEV. It shows no cross-reaction with cdk2/p32 and other cyclin dependent kinases (cdks). Human, mouse, rat, chicken, Xenopus.

    纯化方法

    Purified

    免疫原

    Xenopus p34cdc2.

    亚型

    IgG2a
  • 应用备注

    Approved: Func, IF, IHC, IHC-Fr, IHC-P, IP, WB (2 μg/mL)

    Usage: Suitable for use in Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Western Blot and Kinase Assay. Immunoprecipitation (Native, denatured): 2 μg/mg protein lysate using Protein A. Immunohistochemistry (formalin/paraffin, frozen): 2-4 μg/mL for 30 minutes at RT. Paraffin sections require antigen retrieval. Western Blot: 2 μg/mL for 2 hours at RT. Positive control: HeLa or NIH3T3 cells, germinal center in a tonsil or reactive lymph node.

    说明

    Target Species of Antibody: Xenopus

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeat freeze-thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原

    CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

    别名

    CDK1 / CDC2

    背景

    Name/Gene ID: CDK1
    Subfamily: CDC2/CDK
    Family: Protein Kinase

    Synonyms: CDK1, CDC28A, CDC2, CDKN1, Cyclin-dependent kinase 1, p34 protein kinase, p34CDC2, CDC2a, Cell cycle controller CDC2, Cell division protein kinase 1, PSTAIR

    基因ID

    983

    UniProt

    P06493

    途径

    Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA
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