CD147 抗体 (PE)
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北京 101111
Quick Overview for CD147 抗体 (PE) (ABIN2145003)
抗原
See all CD147 (BSG) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- 4AHIM6 monoclonal antibody reacts with basigin or neurothelin, a 50-60 kDa transmembrane glycoprotein, broadly expressed on cells of hematopoietic and non-hematopoietic origin. Neutrothelin is a blood-brain barrier-specific molecule. CD147 play a role in embryonal blood barrier development and a role in integrin-mediated adhesion in brain endothelia.
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亚型
- IgG1
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anti-Basigin (Ok Blood Group) (BSG) (AA 79-280) antibody
BSG 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Basigin (Ok Blood Group) (BSG) (AA 87-323) antibodyBSG 适用: 小鼠 WB, FACS, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Basigin (Ok Blood Group) (BSG) (AA 59-321) antibodyBSG 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Basigin (Ok Blood Group) (BSG) (AA 30-200) antibodyBSG 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Basigin (Ok Blood Group) (BSG) (AA 138-323) antibodyBSG 适用: 人 FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 2D8C11 unconjugated
anti-Basigin (Ok Blood Group) (BSG) (AA 138-323) antibodyBSG 适用: 人 FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 7H4D4 unconjugated
anti-Basigin (Ok Blood Group) (BSG) (AA 138-323) antibodyBSG 适用: 人 WB, FACS, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Basigin (Ok Blood Group) (BSG) (AA 138-323) antibodyBSG 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 3E10C1 unconjugated
anti-Basigin (Ok Blood Group) (BSG) antibodyBSG 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal BSG-963 unconjugated
anti-Basigin (Ok Blood Group) (BSG) (C-Term) antibodyBSG 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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实验流程
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- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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- CD147 (BSG) (Basigin (Ok Blood Group) (BSG))
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别名
- CD147
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途径
- S100 Proteins
抗原
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