CD9 抗体 (PE)
Quick Overview for CD9 抗体 (PE) (ABIN2144771)
抗原
See all CD9 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
产品特性
- 4AHI9a reacts with CD9 ( p24), a member of the tetraspan ( TM4SF ) family with 24 kDa MW, expressed on platelets and weakly on B-cells. It also expressed on eosinophils, basophils, endothelial and epithelial cells. CD9 antigen modulates cell adhesion, migration and platelet activation. GM1CD9 triggers platelet activation resulted in platelet aggregation, but it is blocked by anti-Fc receptor antibody CD32. This clone is cross reactive with non-human primate.
-
亚型
- IgG1
-
-
anti-CD9 (CD9) (AA 112-195) antibody
CD9 适用: 人 FACS, WB, IHC, ELISA, IF 宿主: 小鼠 Monoclonal 1E8H1 unconjugated
anti-CD9 (CD9) (AA 112-195) antibodyCD9 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 5A6D8 unconjugated
anti-CD9 (CD9) antibodyCD9 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal CD9-1619 unconjugated
anti-CD9 (CD9) antibodyCD9 适用: 人 IHC, StM, ISt 宿主: 小鼠 Monoclonal CD9-2343 unconjugated
anti-CD9 (CD9) (Internal Region) antibodyCD9 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD9 (CD9) antibodyCD9 适用: 人 FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 5G6 unconjugated
anti-CD9 (CD9) (AA 101-200) antibodyCD9 适用: 人, 小鼠, Cow, 大鼠, Pig FACS, WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD9 (CD9) antibody (Biotin)CD9 适用: 小鼠 FACS, WB, IP, ICC 宿主: 大鼠 Monoclonal EM-04 Biotin
anti-CD9 (CD9) antibodyCD9 适用: 人 FACS, WB, IHC (p), MS 宿主: 小鼠 Monoclonal MEM-61 unconjugated
anti-CD9 (CD9) (AA 115-145) antibodyCD9 适用: 人 FACS, WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
实验流程
-
- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C
-
-
- CD9
-
别名
- CD9
-
途径
- Response to Water Deprivation, Cell-Cell Junction Organization
抗原
-
