CD69 抗体 (FITC)
Quick Overview for CD69 抗体 (FITC) (ABIN2144678)
抗原
See all CD69 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- The 4AFN50 monoclonal antibody reacts with human CD69, also known as very eary activation antigen (VEA). CD69 is approximately 30 kDa and is expressed on the cell-surface as a disulfide-linked dimer. CD69 is rapidly upregulated upon activation and expressed on lymphocytes, monocytes and platelets.
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亚型
- IgG1 kappa
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anti-CD69 (CD69) (AA 101-199) antibody
CD69 适用: 人, 小鼠 FACS, WB, ELISA, IHC (fro), IF (p), IF (cc), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD69 (CD69) antibody (FITC)CD69 适用: 人 FACS 宿主: 小鼠 Monoclonal FN50 FITC
anti-CD69 (CD69) antibodyCD69 适用: 人 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 8B6 unconjugated
anti-CD69 (CD69) (AA 1-199) antibodyCD69 适用: 人 FACS, WB 宿主: 小鼠 Polyclonal unconjugated
anti-CD69 (CD69) antibodyCD69 适用: 人 FACS, IHC (fro) 宿主: 小鼠 Monoclonal FN50 unconjugated
anti-CD69 (CD69) antibody (APC)CD69 适用: 人 FACS 宿主: 小鼠 Monoclonal FN50 APC
anti-CD69 (CD69) antibody (PE)CD69 适用: 人 FACS 宿主: 小鼠 Monoclonal FN50 PE
anti-CD69 (CD69) antibody (PE)CD69 适用: 人, Baboon, Cynomolgus, Macaque, 猕猴, Chimpanzee FACS 宿主: 小鼠 Monoclonal FN50 PE
anti-CD69 (CD69) (AA 90-199) antibodyCD69 适用: 人 ELISA, IP 宿主: 小鼠 Monoclonal 4H3 unconjugated
anti-CD69 (CD69) (Internal Region) antibodyCD69 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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实验流程
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- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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- CD69
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别名
- CD69
抗原
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