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CD57 抗体 (FITC)

B3GAT1 适用: 人 FACS, WB, IHC (p), Func 宿主: 小鼠 Monoclonal 4AHNK-1 FITC
产品编号 ABIN2144649
发货至: 中国
  • 抗原 See all CD57 (B3GAT1) 抗体
    CD57 (B3GAT1) (beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1))
    适用
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    宿主
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    小鼠
    克隆类型
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    单克隆
    标记
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    This CD57 antibody is conjugated to FITC
    应用范围
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    Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)
    产品特性
    4AHNK-1 reacts with HNK1 Molecule, a 110 kDa carbohydrate antigen associated with myelin-associated glycoprotein. CD57 expressed on 7-35 % of normal peripheral blood lymphocytes including a subset of naturel killer cells, a subset of CD8+ peripheral blood suppressor / cytotoxic T cells, and on some neural tissues. HNK is not expression on granulocytes, platelets, red blood cells and thymocytes.
    克隆位点
    4AHNK-1
    亚型
    IgM
    Top Product
    Discover our top product B3GAT1 Primary Antibody
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验流程
    • Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
    • Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
    • Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
    • Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
    • [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
    • [Cell fixation: 2 % formaldehyde solution]
    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
  • 抗原
    CD57 (B3GAT1) (beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1))
    别名
    CD57 (B3GAT1 产品)
    别名
    CD57 antibody, GLCATP antibody, GLCUATP antibody, HNK1 antibody, LEU7 antibody, NK-1 antibody, NK1 antibody, 0710007K08Rik antibody, AI846286 antibody, GlcAT-P antibody, Glcatp antibody, Hnk1 antibody, Hnk-1 antibody, b3gat1 antibody, si:dkey-22o20.1 antibody, cd57 antibody, glcatp antibody, glcuatp antibody, hnk-1 antibody, hnk1 antibody, leu7 antibody, nk-1 antibody, nk1 antibody, beta-1,3-glucuronyltransferase 1 antibody, beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) antibody, beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) a antibody, beta-1,3-glucuronyltransferase 1 L homeolog antibody, B3GAT1 antibody, B3gat1 antibody, b3gat1a antibody, b3gat1.L antibody
    途径
    Glycosaminoglycan Metabolic Process
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