CD57 抗体 (FITC)
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北京 101111
Quick Overview for CD57 抗体 (FITC) (ABIN2144649)
抗原
See all CD57 (B3GAT1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- 4AHNK-1 reacts with HNK1 Molecule, a 110 kDa carbohydrate antigen associated with myelin-associated glycoprotein. CD57 expressed on 7-35 % of normal peripheral blood lymphocytes including a subset of naturel killer cells, a subset of CD8+ peripheral blood suppressor / cytotoxic T cells, and on some neural tissues. HNK is not expression on granulocytes, platelets, red blood cells and thymocytes.
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亚型
- IgM
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anti-beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1) antibody (PE)
B3GAT1 适用: 人 FACS 宿主: 小鼠 Monoclonal TB01 PE
anti-beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1) (AA 28-334) antibodyB3GAT1 适用: 人 FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 1A4F10 unconjugated
anti-beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1) (AA 35-191) antibodyB3GAT1 适用: 人, 小鼠, 大鼠, 猴 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 3F6D6 unconjugated
anti-beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1) (AA 193-334) antibodyB3GAT1 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 3B8B10 unconjugated
anti-beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1) antibody (APC)B3GAT1 适用: 人 FACS 宿主: 小鼠 Monoclonal TB01 APC
anti-beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1) (AA 21-120) antibodyB3GAT1 适用: 人, 小鼠, 大鼠 FACS, WB, IHC (p), ELISA, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1) antibodyB3GAT1 适用: 人 FACS, WB, IHC (p), ICC, IHC (fro) 宿主: 小鼠 Monoclonal TB01 unconjugated
anti-beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1) antibody (Biotin)B3GAT1 适用: 人 FACS, IHC, IP 宿主: 小鼠 Monoclonal NK-1 Biotin
anti-beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1) antibody (FITC)B3GAT1 适用: 人 FACS, IHC 宿主: 小鼠 Monoclonal NK-1 FITC
anti-beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1) antibody (SPRD)B3GAT1 适用: 人 FACS, IHC 宿主: 小鼠 Monoclonal NK-1 SPRD
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应用备注
- Optimal working dilution should be determined by the investigator.
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实验流程
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- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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- CD57 (B3GAT1) (beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1))
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别名
- CD57
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途径
- Glycosaminoglycan Metabolic Process
抗原
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