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CD45RA 抗体 (FITC)

This anti-CD45RA antibody (ABIN2144603) is a Mouse Monoclonal antibody detecting CD45RA in FACS, IF, IHC (p), WB. Suitable for Human.
产品编号 ABIN2144603
发货至: 中国

Quick Overview for CD45RA 抗体 (FITC) (ABIN2144603)

抗原

See all CD45RA 抗体
CD45RA

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This CD45RA antibody is conjugated to FITC

应用范围

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Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

克隆位点

4AHI100
  • 产品特性

    4AHI100 reacts with the 220 kDa isoform A of CD45. This is clustered as CD45RA, and is expressed on naive/resting T cells and on medullart thymocytes. In comparison, CD45RO is expressed on memory/activated T cells and cortical thymocytes. CD45RA and CD45RO are useful for discriminating between naive and memory T cells in the study of the immune system.

    亚型

    IgG2b
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    实验流程

    • Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
    • Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
    • Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
    • Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
    • [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
    • [Cell fixation: 2 % formaldehyde solution]

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C
  • 抗原

    CD45RA

    别名

    CD45RA
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