Fc gamma RII (CD32) 抗体 (FITC)
Quick Overview for Fc gamma RII (CD32) 抗体 (FITC) (ABIN2144558)
抗原
See all Fc gamma RII (CD32) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- 4AIV-3 reacts with an low affinity receptor for aggregated IgG (FcgRII), 40 kD. CD32 Molecule is expressed on B cells, monocytes, granulocytes and platelets. This clone also cross-reacts with monocytes, granulocytes and subset of peripheral blood lymphocytes of non-human primates.The reactivity on leukocyte populations is similar to that Obs.
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亚型
- IgG2a
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anti-Fc gamma RII (CD32) antibody
CD32 适用: 人 FACS, WB, IHC, IP, IHC (p) 宿主: 小鼠 Monoclonal 6D11 unconjugated
anti-Fc gamma RII (CD32) (AA 201-300) antibodyCD32 适用: 人 FACS, WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Fc gamma RII (CD32) antibodyCD32 适用: 人 FACS, MS 宿主: 小鼠 Monoclonal 3D3 unconjugated
anti-Fc gamma RII (CD32) antibody (PE)CD32 适用: 人 FACS 宿主: 小鼠 Monoclonal 3D3 PE
anti-Fc gamma RII (CD32) (AA 69-309) antibodyCD32 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fc gamma RII (CD32) antibody (PE)GMP Grade CD32 适用: 人 FACS 宿主: 小鼠 Monoclonal IV-3 PE
anti-Fc gamma RII (CD32) antibody (FITC)GMP Grade CD32 适用: 人 FACS 宿主: 小鼠 Monoclonal IV-3 FITC
anti-Fc gamma RII (CD32) (AA 36-229) antibodyCD32 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Fc gamma RII (CD32) antibodyCD32 适用: 人, 犬, 猕猴, Pig FACS, IP, Func, IHC (fro) 宿主: 小鼠 Monoclonal AT10 unconjugated
anti-Fc gamma RII (CD32) antibody (PE)CD32 适用: Cow, 绵羊 FACS 宿主: 小鼠 Monoclonal CCG36 PE
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应用备注
- Optimal working dilution should be determined by the investigator.
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实验流程
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- Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
- Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
- Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
- Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
- Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
- [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
- [Cell fixation: 2 % formaldehyde solution]
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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- Fc gamma RII (CD32)
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别名
- CD32
抗原
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